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Series GSE96807 Query DataSets for GSE96807
Status Public on Mar 26, 2017
Title Genome-wide profiling of genes during differentiation of wild (WT) murine embryonic stem cells (ESCs), scrambled control (SCR) ESCs, and Strip2 silenced (KD) ESCs
Organism Mus musculus
Experiment type Expression profiling by array
Summary The role of Striatin Interacting Protein 2 (Strip2) in differentiation of embryonic stem cells (ESCs) is still under debate. Strip2 silenced (KD) ESCs were differentiated for 4, 8, 12, and 16 days. We show that Strip2 is distributed in the perinucleus or nuclei of wild type (WT) undifferentiated ESCs, but is localized in high-density nuclear bodies in differentiated cells. CellNet analysis of microarray gene expression data for KD and scrambled control (SCR) embryoid bodies (EBs), as well as immunostainings of key pluripotent factors, demonstrated that KD ESCs remain undifferentiated. This occurs even in 16-day old EBs, which possessed a high tumorigenic potential. Correlated with very high expression levels of epigenetic regulator genes, Hat1 and Dnmt3, enzymatic activities of the histone acetyltransferase type B (HAT1) and DNA (cytosine-5)-methyltransferase 3 beta (DNMT3b) were higher in differentiated 16-day old KD EBs than in SCR or WT EBs. The expression levels of let-7, 290 and 302 microRNA families were opposed in KD ESCs, while KD EBs had levels comparable to WT and SCR ESCs during differentiation. This demonstrates that Strip2 is critical to the onset of differentiation, regulating expression of epigenetic regulators, HAT1 and DNMT3b, as well as microRNAs involved in pluripotency.
Overall design RNA from undifferentiated WT ESCs, SCR ESCS and KD ESCs as well as of 4-, 8-, 12- and 16-day EBs was isolated and gene expression was analysed by the Affymetrix (Mouse 430 2.0) chip
Contributor(s) Sachinidis A
Citation(s) 28480311
Submission date Mar 20, 2017
Last update date Feb 11, 2019
Contact name Agapios Sachinidis
Phone +492214787373
Organization name University of Cologne
Department Institute of Neurophysiology
Street address Robert-Kochstr. 39
City Cologne
State/province NRW
ZIP/Postal code 50931
Country Germany
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (45)
GSM2544350 WT_ES_3
GSM2544351 WT_D16_3
GSM2544352 WT_D12_3
This SubSeries is part of SuperSeries:
GSE96809 Genome-wide profiling of genes and miRNAs during differentiation of wild (WT) murine embryonic stem cells (ESCs), scrambled control (SCR) ESCs, and Strip2 silenced (KD) ESCs
BioProject PRJNA379783

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE96807_RAW.tar 153.0 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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