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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 18, 2017 |
| Title |
Epithelial-Mesenchymal Micro-Niches Govern Stem Cell Lineage Choices (ATAC-seq) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Adult tissue stem cells (SCs) reside in niches, which through intercellular contacts and signaling, influence SC behavior. Once activated, SCs typically give rise to short-lived transit-amplifying cells (TACs), which then progress to differentiate into their lineages. Here, using single cell RNA-sequencing, we unearth unexpected heterogeneity among SCs and TACs of hair follicles. We trace the roots of this heterogeneity to micro-niches along epithelial-mesenchymal interfaces, where progenitors display molecular signatures reflective of spatially distinct local signals and intercellular interactions. Using lineage-tracing, temporal single cell analyses and chromatin landscaping, we show that SC plasticity becomes restricted in a sequentially and spatially choreographed program, culminating in seven spatially arranged uni-lineage progenitors within TACs of mature follicles. By compartmentalizing SCs into micro-niches, tissues gain precise control over morphogenesis and regeneration: Some progenitors specify lineages immediately; others retain potency, preserving self-renewing features established early while progressively restricting lineages as they experience dynamic changes in microenvironment.
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| Overall design |
Mouse bulge hair follicle stem cell and hair germ cells at telogen stage were FACS-purified and analyzed by ATAC-seq
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| Contributor(s) |
Yang H, Fuchs E |
| Citation(s) |
28413068 |
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| Submission date |
Mar 17, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Hanseul Yang |
| E-mail(s) |
hyang01@rockefeller.edu
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| Organization name |
Rockefeller University
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| Lab |
Fuchs lab
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| Street address |
1230 York Avenue
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE90850 |
Epithelial-Mesenchymal Micro-Niches Govern Stem Cell Lineage Choices |
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| Relations |
| BioProject |
PRJNA379620 |
| SRA |
SRP102095 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE96782_RAW.tar |
3.7 Mb |
(http)(custom) |
TAR (of NARROWPEAK) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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