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Series GSE9673 Query DataSets for GSE9673
Status Public on Nov 22, 2008
Title Comparative transcriptomic reveals signatures specific to various interactions with Melampsora rust fungi
Platform organism Populus sp.
Sample organism Populus maximowiczii x Populus nigra
Experiment type Expression profiling by array
Summary We aim to understand the global transcriptional response of hybrid poplar NM6 (Populus nigra x Populus maximoviczii) to infection by two biotrophic Melampsora fungi, M. larici-populina and M. medusae f. sp. deltoidae separately and simultanously. Transcript profilling revealed 699 differentially expressed genes whose expression level was equal or higher than 2-fold between infected and noninfected tissues. In the mixed infection, our results showed an additive response of the two pathogens.
Genes regulated as a result of Melampsora infection were generally induced and mainly involved in primary/secondary metabolism that include cell wall reinforcement and lignification, defense-related processes like pathogenesis-related and stress response, signal perception and transduction mechanisms and regulation of transcription.
Keywords: Stress response
 
Overall design Poplar leaves were infected with Melampsora larici-populina and Melampsora medusae f. sp. deltoidae and the mixture of them (Mixed infection). At six day post-infection, tissues were harvested for RNA extraction and hybridization on Affymetrix microarrays. Three biological replications were obtained from infected and non infected leaves (control samples).
 
Contributor(s) Azaiez A, Levée V, Boyle B, Séguin A
Citation(s) 19132871
Submission date Nov 23, 2007
Last update date Jan 02, 2015
Contact name Armand Séguin
E-mail(s) Armand.Seguin@rncan.gc.ca
Phone 418-648-2533
Organization name Laurentian forestry Service
Street address 1055 rue de PEPS
City Québec
State/province Québec
ZIP/Postal code G1V4C7
Country Canada
 
Platforms (1)
GPL4359 [Poplar] Affymetrix Poplar Genome Array
Samples (12)
GSM244430 non infected tissue, biological rep1
GSM244431 non infected tissue, biological rep2
GSM244432 non infected tissue, biological rep3
Relations
BioProject PRJNA103559

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9673_RAW.tar 51.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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