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Series GSE96586 Query DataSets for GSE96586
Status Public on Jun 13, 2018
Title Murine Norovirus Infection Initiates Interferon and TNF Signaling with Significant Alteration of Lipid and Cell Cycle Homeostasis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Murine norovirus (MNV) is genetically similar to human norovirus (HuNoV), and offers both an efficient in vitro cell culture system and animal model by which to investigate the molecular basis of replication. Here, we present a detailed global view of the cellular alterations that occur during the progression of a norovirus infection. The transcriptome of a synchronously infected population of MNV-infected murine macrophage-like cell line (RAW264.7) was determined at 8, 14, and 20 hours post infection. The cellular genetic response was analyzed both globally and by specific pathways. Viral replication was monitored by RNA-seq and quantitative real-time PCR in context of the cellular phenotypic response. The majority of transcriptionally up regulated genes were related to the IFN response. Additionally, there was a global increase in gene transcripts associated with immune response and inflammation. A transcriptional decrease was observed across many cellular processes, but particularly for genes involved in lipid homeostasis and cell cycle. The peak of the transcriptional immune response correlated with detected viral genome copies and changes in cellular phenotype including nuclear condensation. A more complete understanding of host response to norovirus infection will help to highlight the cellular pathways critical for a more effective immune response as well as those that may be exploited by the virus for therapeutic development.
 
Overall design Time course study of murine macrophage-like cell line gene expression infected with Murine nororvirus.
 
Contributor(s) Levenson EA, Martens C, Kanakabandi K, Turner C, Sosnovtsev SV, Ricklefs S, Johnson JA, Porcella SF, Green KY
Citation(s) 29735480
Submission date Mar 14, 2017
Last update date May 15, 2019
Contact name Craig Martens
E-mail(s) martensc@niaid.nih.gov
Phone 406-363-9415
Organization name NIAID
Department RTB
Street address 903 S. 4th St
City Hamilton
State/province MT
ZIP/Postal code 59840
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (16)
GSM2536263 MNV0-1
GSM2536264 MNV0-2
GSM2536265 MNV0-3
This SubSeries is part of SuperSeries:
GSE111663 Comparative transcriptomic response of primary and immortalized macrophage to murine norovirus infection
Relations
BioProject PRJNA379104
SRA SRP101852

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Supplementary file Size Download File type/resource
GSE96586_HS83_normalized_counts.txt.gz 1.4 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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