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| Status |
Public on Aug 17, 2017 |
| Title |
Transcrptional profiles of Cryptococcous neoformans Gsb1 at non-stress and oxidative stress conditions |
| Organism |
Cryptococcus neoformans var. grubii H99 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: To identify the downstream genes whose expression levels are affected by the deletion of gsb1 under non-stress and oxidative stress conditions, we compared the RNA-seq based transcriptional profiles of wild-type and gsb1 cells.
Methods: mRNA profiles of cells grown at non-stress and oxidative stress conditions were generated by RNA-seq using Illumina GAIIx. The sequence reads that passed quality filters were mapped to reference genome and the normalized RPKM values were calculated by CLC Genomics Workbench.
Results: RNA-Seq analysis during normal growth revealed increased expression of a number of genes involved in mitochondrial respiration and cell cycle, but decreased expression of several genes involved in the mating-responsive MAPK signaling pathway. Moreover, RNA-Seq analysis under oxidative stress revealed that several genes involved in ROS defense, cell wall remodeling, and protein glycosylation were highly induced in the wild-type strain but not in gsb1.
Conclusions: This study proposed that Gsb1 is a key transcriptional regulator modulating growth, anti-stress responses, differentiation, and virulence in C. neoformans.
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| Overall design |
mRNA profiles of cells grown at non-stress and oxidative stress conditions were generated by RNA-seq using Illumina.
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| Contributor(s) |
Cheon SA, Kang HA |
| Citation(s) |
28642475 |
| |
| Submission date |
Mar 13, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Seon Ah Cheon |
| E-mail(s) |
chnamoo7@naver.com
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| Organization name |
Chung-Ang University
|
| Street address |
84 Heukseok-ro
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| City |
Seoul |
| ZIP/Postal code |
06974 |
| Country |
South Korea |
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| Platforms (2) |
| GPL15546 |
Illumina Genome Analyzer IIx (Cryptococcus neoformans var. grubii H99) |
| GPL23176 |
Illumina HiSeq 2500 (Cryptococcus neoformans var. grubii H99) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA378989 |
| SRA |
SRP101795 |
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