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Series GSE95712 Query DataSets for GSE95712
Status Public on Aug 31, 2017
Title Bmal1 regulates inflammatory response in macrophage
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Deletion of Arntl disrupted temporal inflammatory response in macrophage. Mechanistically, the acetylation status of lysine 27 of histone 3 (H3K27ac) was enhanced at the PU.1-containing enhancers in Arntl-/- macrophages compared to the wild-type cells. Collectively, transcription factor network containing Bmal1 controls temporal inflammatory response of macrophages by regulating epigenetic states of enhancers.
Overall design Genome wide localization of Transcription factor Bmal1, NFkB (p65) and acetylation status of lysine 27 of histone 3 (H3K27ac) were assessed by ChIP-Seq. Genome-wide gene expression were analyzed by RNA-seq at indicated timepoints in the course of inflammatory response in primary macrophages.
Contributor(s) Oishi Y
Citation(s) 28765524, 32513690
Submission date Mar 06, 2017
Last update date Jul 08, 2020
Contact name Yumiko Oishi
Organization name Tokyo Medical and Dental University
Department Department of Cellular and Molecular Medicine
Street address 1-5-45, Yushima
City Bunkyo
State/province Tokyo
ZIP/Postal code 113-8510
Country Japan
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (37)
GSM2522445 PolyA RNA-seq BmalKO KLA0h
GSM2522446 PolyA RNA-seq BmalKO KLA6h
GSM2522447 PolyA RNA-seq BmalKO KLA24h
BioProject PRJNA378204
SRA SRP101430

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE95712_BMDM-FPKM.txt.gz 1.7 Mb (ftp)(http) TXT
GSE95712_RAW.tar 3.0 Gb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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