GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE95665 Query DataSets for GSE95665
Status Public on Mar 26, 2018
Title Arabidopsis ZED1-related kinases mediate temperature-sensitive intersection of immune response and growth homeostasis
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary The rationale: Activation of the immune response antagonizes plant growth and development in absence of pathogen, and such autoimmune phenotype is often suppressed by the elevation of ambient temperature. However, the molecular regulation of ambient temperature-sensitive intersection of immune response and growth is largely elusive.
Methods: A genetic screen identified an Arabidopsis mutant, zed1-D, by its high temperature-dependent growth retardation. A combination of molecular, cytological and genetic approaches was used to investigate the molecular basis behind the temperature-sensitive growth and immune response in zed1-D.
Key results: A dominant mutation in HOPZ-ETI-DEFICIENT 1 (ZED1) is responsible for a high temperature-dependent autoimmunity and growth retardation in the zed1-D. The autoimmune phenotype in the zed1-D is dependent on the HOPZ-ACTIVATED RESISTANCE 1 (ZAR1). ZED1 and some ZED1-related kinases (ZRKs) are induced by elevated temperature and function cooperatively to suppress the immune response by modulating the transcription of SUPPRESSOR OF NPR1-1 CONSTITUTIVE 1 (SNC1) in absence of pathogen.
Main conclusion: Our data reveal a previously unidentified role of ZRKs in ambient temperature-sensitive immune response in absence of pathogen, and thus disclose a possible molecular mechanism underlying temperature-mediated intersection of immune response and growth in plants.
Overall design Compared the transcriptome of zed1-D with WT plants after being transferred from 18 into 28oC at different time points, 0h, 3h, 12h, and 48h.
Contributor(s) Wang Z, Cui D, Hu Y
Citation(s) 28499073
Submission date Mar 03, 2017
Last update date May 15, 2019
Contact name Wang Zhicai
Organization name Institute of Botany, Chinese Academy of Sciences
Street address No.20 Nanxincun, Xiangshan
City Beijing
ZIP/Postal code 100093
Country China
Platforms (1)
GPL13222 Illumina HiSeq 2000 (Arabidopsis thaliana)
Samples (16)
GSM2520860 WT 0h R1
GSM2520861 WT 0h R2
GSM2520862 WT 3h R1
BioProject PRJNA377889
SRA SRP101351

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE95665_RAW.tar 4.2 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap