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Series GSE95643 Query DataSets for GSE95643
Status Public on Mar 02, 2018
Title CARM1-expressing ovarian cancer depends on the histone methyltransferase EZH2 activity [ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary CARM1 is an arginine methyltransferase that asymmetrically dimethylates protein substrates on arginine residues. CARM1 is often overexpressed in cancers and stimulates growth. However, clinically applicable therapeutic strategies based on CARM1 expression in cancer remains to be explored. Here we show that epithelial ovarian cancer is among the cancers with the highest CARM1 amplification rates that predicates a shorter survival. Our unbiased screen show that CARM1-expressing ovarian cancer cells are selectively sensitive to the inhibition of EZH2, another epigenetic regulator that silences its target genes. Inhibition of EZH2 activity using a clinically applicable small molecule inhibitor significantly suppressed the growth of CARM1-expressing ovarian tumors in two xenograft models. The observed selectivity correlates with upregulation of EZH2 target genes in a CARM1-dependent manner. CARM1 promotes EZH2 dependent gene silencing by methylating BAF155 to alter the antagonism between EZH2 and BAF155. Together, these results indicate that pharmacological inhibition of EZH2 is a novel therapeutic strategy for CARM1-expressing cancers.
 
Overall design CARM1 wild type and knockout samples assayed by EZH2 and H3K27me3 CHIP-seq
Web link https://www.ncbi.nlm.nih.gov/pubmed/?term=CARM1-expressing+ovarian+cancer+depends+on+the+histone+methyltransferase+EZH2+activity
 
Contributor(s) Zhang R, Karakashev S
Citation(s) 29434212
Submission date Mar 02, 2017
Last update date May 15, 2019
Contact name Priyankara J Wickramasinghe
E-mail(s) priyaw@wistar.org
Phone 2154956837
Organization name The Wistar Institute
Department Bioinformatics
Lab Genomics
Street address 3601 Spruce Street
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (6)
GSM2520571 CHIP.CARM1-WT.EZH2
GSM2520572 CHIP.CARM1-WT.H3K27me3
GSM2520573 CHIP.CARM1-WT.input
This SubSeries is part of SuperSeries:
GSE95645 CARM1-expressing ovarian cancer depends on the histone methyltransferase EZH2 activity
Relations
BioProject PRJNA377753
SRA SRP101295

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE95643_CARM1_CHIPseq_EZH2.KOvsWT.txt.gz 677.8 Kb (ftp)(http) TXT
GSE95643_CARM1_CHIPseq_EZH2.WTvsKO.txt.gz 372.8 Kb (ftp)(http) TXT
GSE95643_CARM1_CHIPseq_H3K27me3.KOvsWT.txt.gz 946.6 Kb (ftp)(http) TXT
GSE95643_CARM1_CHIPseq_H3K27me3.WTvsKO.txt.gz 1.5 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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