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Series GSE95531 Query DataSets for GSE95531
Status Public on Apr 26, 2017
Title Lineage specific differentiation is influenced by state of human pluripotency [methylation]
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary Human pluripotent stem cells (hPSCs) have been reported in naïve and primed states. However, the ability of human PSCs to generate mature cell types is the only imperative property for translational utility. Here, we reveal that the naïve state enhances self-renewal capacity while restricting lineage differentiation in vitro to neural default fate. Gene expression analyses indicate expression of multiple lineage associated transcripts in naïve hPSCs and thus failed to predict biased functional differentiation. Naïve hPSCs can be converted to primed allowing recovery of multilineage differentiation over long serial passage or immediately through suppression of OCT4 but not NANOG. To this end, we identified chemical inhibitors of OCT4 expression that acutely restore naïve hPSC differentiation. Our study identifies unique cell fate features and critical restrictions in human pluripotent states, and provides an approach to overcome these barriers that harness both efficient naïve hPSC growth whilst maintaining in vitro differentiation capacities essential for hPSC applications.
 
Overall design hPSC lines were transduced with shRNA lentiviruses in order to assess the effects of reducing NANOG and OCT4 gene expression on differention in the naïve state. shRNA expressing cells were sorted and then genomic DNA was extracted in order to assess methylation status.
 
Contributor(s) Lee JH, Bhatia M
Citation(s) 28380358
Submission date Feb 28, 2017
Last update date Jan 20, 2019
Contact name Mickie Bhatia
E-mail(s) mbhatia@mcmaster.ca
Organization name McMaster University
Department Stem Cell and Cancer Research Institute (SCC-RI)
Street address 1200 Main Street West
City Hamilton
State/province Ontario
ZIP/Postal code L8N 3Z5
Country Canada
 
Platforms (1)
GPL21145 Infinium MethylationEPIC
Samples (8)
GSM2515899 human pluripotent stem cells, primed, control, 1
GSM2515900 human pluripotent stem cells, primed, control, 2
GSM2515901 human pluripotent stem cells, naïve, control, 1
This SubSeries is part of SuperSeries:
GSE95505 Lineage specific differentiation is influenced by state of human pluripotency
Relations
BioProject PRJNA377404

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE95531_Matrix_signal_intensities_JH_LIF2i_2017_Methylation_.txt.gz 42.4 Mb (ftp)(http) TXT
GSE95531_RAW.tar 273.5 Mb (http)(custom) TAR (of IDAT)
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Raw data is available on Series record
Raw data provided as supplementary file
Processed data included within Sample table

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