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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jun 13, 2017 |
| Title |
Expression regulation by Pcgf1 in mouse embryonic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The Polycomb repressive complex 1 (PRC1) is essential for fate decisions of embryonic stem (ES) cells. Emerging evidence suggests that six major variants of PRC1 complex, defined by the mutually exclusive presence of Pcgf subunit, regulate distinct biological processes, yet very little is known about the mechanism by which each version of PRC1 instructs and maintains cell fate. Here, we disrupted the Pcgf1, also known as Nspc1 and one of six Pcgf paralogs, in mouse ES cells by the CRISPR/Cas9 technology. We showed that although these mutant cells were viable and retained normal self-renewal, they displayed severe defects in differentiation in vitro. To gain a better understanding of the role of Pcgf1 in transcriptional control of differentiation, we analysed mRNA profiles from Pcgf1 deficient cells using RNA-seq. In contrast to the canonical role of PRC1 in gene repression, we found that Pcgf1 mainly functioned as a transcriptional activator to drive expression of many genes involved in ectoderm and mesoderm differentiation. Chromatin immunoprecipitation experiments demonstrated that Pcgf1 deletion caused a decrease in Ring1B and its associated H2AK119ub1 mark binding to target genes. Altogether, our results revealed an unexpected function of Pcgf1 in gene activation during ES cell maintenance.
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| Overall design |
Pcgf1 KO, wildtype and Pcgf1KO+ Pcgf1_flag mESCs
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| Contributor(s) |
Yan Y, Zhao W, Qin J |
| Citation(s) |
28393894 |
| BioProject |
PRJNA374775 |
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| Submission date |
Feb 26, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Wukui Zhao |
| E-mail(s) |
zhaowukui163@163.com
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| Phone |
86-02558641524
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| Organization name |
Nanjing University
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| Department |
Model Animal Research Center
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| Lab |
MOE Key Laboratory of Model Animal for Disease Study
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| Street address |
12 Xuefu Road
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| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
210061 |
| Country |
China |
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| Platforms (1) |
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| Samples (3) |
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| Relations |
| SRA |
SRP099821 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE95383_RAW.tar |
7.9 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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