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| Status |
Public on May 02, 2017 |
| Title |
Poly(A)-ClickSeq resolves CF25-mediated alternative poly-adenylation, HeLa |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Poly(A)-ClickSeq is a new methodology for the sequencing of the 3'UTR/poly(A) tail junction of RNA. We analysed both wild-type and CF25Im knock-down HeLa cells in culture using Poly(A)-ClickSeq to find the distribution of poly(A) sites in these samples and determine the role of CF25Im in poly(A) site selection (alternative poly-adenylation, APA).
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| |
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| Overall design |
RNA was extracted from HeLa cells with or without CF25Im Knock-down, each in triplicates, and sequenced using Poly(A)-ClickSeq and sequencing once on a HiSeq and once on a MiSeq
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| |
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| Contributor(s) |
Routh A, Wagner E, Ji P, Xia Z |
| Citation(s) |
28449108 |
| |
| Submission date |
Feb 15, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Zheng Xia |
| E-mail(s) |
xiaz@ohsu.edu
|
| Organization name |
Oregon Health & Science University
|
| Department |
Department of Biomedical Engineering
|
| Lab |
Xia Lab
|
| Street address |
3181 SW Sam Jackson Park Rd
|
| City |
Portland |
| State/province |
Oregon |
| ZIP/Postal code |
97239 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL15520 |
Illumina MiSeq (Homo sapiens) |
| GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA374982 |
| SRA |
SRP100048 |
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