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Series GSE94682 Query DataSets for GSE94682
Status Public on Sep 05, 2017
Title Endogenous Androgen Receptor proteomic profiling reveals genomic subcomplex involved in prostate tumorigenesis
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Androgen Receptor (AR) is a key player in prostate cancer development and progression. Here, we applied immunoprecipitation mass spectrometry of endogenous AR in LNCaP cells to identify individual components of the AR transcription complex. In total, 66 known and novel AR interactors were identified in the presence of R1881, which were critically and selectively required in AR-driven prostate cancer cell proliferation. AR interactors required for LNCaP proliferation were profiled using ChIP-seq, identifying distinct genomic subcomplexes of AR interaction partners. Interestingly, three major subgroups of genomic subcomplexes were identified, where a selective gain-of-function for AR was identified in tumorigenesis, dictated by FOXA1 and HOXB13. Jointly, our study reveals subclasses of the AR transcription complex formation and composition, that is dictated by overexpression of AR-interactors, resulting in reprogramming of the AR cistrome and interactome in a genomic location-specific manner.
 
Overall design AR, ARID1a, BRG1, FOXA1, HOXB13, TLE3, TRIM28 and WDHD1 binding to DNA was profiled by ChIP-seq (Chromatin Immunoprecipitation) genome-wide in prostate cancer cell line LNCaP.
 
Contributor(s) Stelloo S, Zwart W
Citation(s) 28925401
Submission date Feb 08, 2017
Last update date Sep 30, 2019
Contact name Wilbert Zwart
E-mail(s) w.zwart@nki.nl
Organization name Netherlands Cancer Institute
Department Molecular Pathology
Street address Plesmanlaan 121
City Amsterdam
ZIP/Postal code 1066 CX
Country Netherlands
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (34)
GSM2480800 AR_veh_repl1
GSM2480801 AR_r1881_repl1
GSM2480802 AR_veh_repl2
Relations
BioProject PRJNA371800
SRA SRP099063

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE94682_RAW.tar 9.6 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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