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Series GSE94638 Query DataSets for GSE94638
Status Public on Mar 27, 2017
Title Gene expression in germinal centre light zone and dark zone cells of high or low affinity
Organism Mus musculus
Experiment type Expression profiling by array
Summary Gene expression analysis performed on FACS sort purified GC LZ and DZ cells of either high or low affinity to identify unique gene signatures.
Analysis revealed distinct transcription patterns.
 
Overall design SwHEL B cells carrying the CD45.1 congenic marker were transferred into C57BL/6J mice (CD45.2) and immunized with sheep red blood cells conjugated to the hen egg lysozyme mutant HEL3x. Day 10 post immunization high and low affinity cells of either LZ (CD86 high, CXCR4 low) or DZ (CXCR4 high, CD86 low) were sort purified from donor-derived GC B cell population (CD45.1+, CD45.2-, IgG1+, CD38 low).
 
Contributor(s) Krautler NJ, Kaplan W, Brink R
Citation(s) 28363897
Submission date Feb 07, 2017
Last update date Mar 04, 2019
Contact name Nike Krautler
E-mail(s) knike@ethz.ch
Organization name ETH Zurich
Department Biology, Institute of Microbiology
Lab Infection Immunology
Street address Vladimir-Prelog Weg1-5/10
City Zurich
ZIP/Postal code 8093
Country Switzerland
 
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (15)
GSM2479961 Dzhi replicate 1
GSM2479962 Dzhi replicate 2
GSM2479963 Dzhi replicate 3
Relations
BioProject PRJNA371646

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE94638_RAW.tar 58.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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