|
| Status |
Public on Apr 19, 2017 |
| Title |
Autonomous TNF is critical for monocyte survival in steady state and inflammation in vivo |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Monocytes are circulating mononuclear phagocytes, poised to extravasate to sites of inflammation and differentiate into monocyte-derived macrophages and dendritic cells. Tumor necrosis factor (TNF) and its receptors are upregulated during monopoiesis and expressed by circulating monocytes, as well as effector monocytes infiltrating certain sites of inflammation, such the spinal cord during experimental autoimmune encephalomyelitis (EAE). Using competitive in vitro and in vivo assays, we show here that monocytes deficient for TNF or TNF receptors are outcompeted by their wild-type counterpart. Moreover, monocyte autonomous TNF is critical for the function of these cells, as TNF ablation in monocytes / macrophages, but not in microglia delayed the onset of EAE in challenged animals and was associated with reduced acute spinal cord infiltration of Ly6Chi effector monocytes. Collectively, our data reveal a previously unappreciated critical cell-autonomous role of TNF on monocytes for their survival, maintenance and function.
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| Overall design |
BM precursors and steady state monocytes are taken and compared with effector spinal cord monocytes in EAE day 9
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| |
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| Contributor(s) |
Wolf Y, Mildner A, Jung S |
| Citation(s) |
28330904 |
| |
| Submission date |
Feb 06, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Steffen Jung |
| E-mail(s) |
s.jung@weizmann.ac.il
|
| Phone |
0097289342787
|
| Organization name |
The Weizmann Institute of Science
|
| Department |
Immunology and Regenerative Biology
|
| Lab |
Steffen Jung
|
| Street address |
Herzl
|
| City |
REHOVOT |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA371454 |
| SRA |
SRP098904 |
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