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Series GSE94370 Query DataSets for GSE94370
Status Public on Apr 19, 2017
Title Effect of ZincOxide in Weaned Piglets
Organism Sus scrofa
Experiment type Expression profiling by array
Summary Development of immune competence in pigs is important for resilience, disease resistance, and performance later in life. Dietary interventions in early life can contribute to immune system development. Use of model dietary interventions, such as diets with a high concentration of zinc, can contribute to the understanding of the interactions between the diet, the intestinal microbiota and intestinal tissues. The aim of the present study was to evaluate the effects of provision of a diet with a high concentration of zinc (Zn, 2690 mg/kg), as model intervention, as compared to provision of a control diet with a regular Zn concentration (100 mg/kg) during a period of nine days during the post weaning period (d 14-23 post weaning (pw)), on the composition of the intestinal microbiota and gene expression in the intestinal mucosa on d 23 and 35 pw (12 d after the termination of the Zn intervention). Whole genome gene expression analysis of the jejunal and ileal mucosa revealed a small overall time-treatment interaction effect at d 23 pw in both intestinal tissues. In both jejunal and ileal mucosa various genes/probes were differentially up or down regulated between treatments. In intestinal tissue samples obtained on d 35 no differences in gene expression were observed. Probes of d 23 pw that were differently expressed and had an annotation were subsequently used as input for further functional analysis using DAVID and Gene Decks databases. The analysis did not result in the identification of gene sets that were differentially expressed between dietary treatments. However, it was shown that a number of upregulated genes in the jejunal mucosa on d 23 pw by the high zinc intervention are involved in pathways related to mineral absorption, immune signalling and cell energy metabolism (glycolysis and gluconeogenesis). A few down regulated genes by the Zn intervention are also involved in immune signalling pathways. It was concluded from the present study that provision of a diet with a high concentration of zinc as zinc oxide during a short period of time (9 days) to piglets in the post weaning period (d 14-23 pw) induces differences on the intestinal expression of genes in part related to the functioning of the local innate immune system. The high dietary zinc intervention can therefore be considered as a suitable model for studying relationships between dietary interventions and development of immune competence in post weaning piglets.
Overall design In total, 36 piglets were euthanised. i.e.6 pigs per time-point per treatment, 3 time-point; age d42, d51, and d63 (18 pigs), both control and treatment samples (36 pigs)
Contributor(s) Schokker D, Jansman A, Vastenhouw S
Citation(s) 28413565
Submission date Feb 01, 2017
Last update date Apr 21, 2017
Contact name Dirkjan Schokker
Organization name Wageningen UR
Department Wageningen Livestock Research
Street address Droevendaalsesteeg 1
City Wageningen
ZIP/Postal code 6708 PB
Country Netherlands
Platforms (1)
GPL18045 Agilent-035953 Sus scrofa Array
Samples (55)
GSM2474168 ileum_Control_d42_f_2_798
GSM2474169 ileum_High Zinc_d42_m_2_798
GSM2474170 ileum_Control_d42_m_1_798
BioProject PRJNA369539

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Supplementary file Size Download File type/resource
GSE94370_RAW.tar 120.5 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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