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Series GSE94322 Query DataSets for GSE94322
Status Public on Mar 23, 2017
Title A family of double-homeodomain transcription factors promotes zygotic genome activation in placental mammals [ChIP-seq_d1]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary In metazoans, the fertilized embryo is transcriptionally mostly silent for a few cell divisions, until release of a first major wave of embryonic transcripts by so-called zygotic genome activation (ZGA). Maternally provided ZGA-triggering factors have been identified in Drosophila melanogaster and Danio rerio but their mammalian homologues are still undefined. Here, we reveal that DUX family transcription factors are key to this process in human and mouse. First, human Dux4 and murine Dux are expressed prior to ZGA in both species. Second, both orthologues bind to and activate the promoters of ZGA genes. Third, Dux knockdown hinders the progression of mouse embryos beyond the 2-cell stage. Fourth, it prevents the cycling of mouse embryonic stem (ES) cells through a 2-cell-like state whereas, conversely, Dux overexpression induces the accumulation of these ZGA genes-expressing ES cells. We conclude that DUX proteins are master regulators of mammalian ZGA.
Overall design ChIP-sequencing analysis of DUX4 ectopically expressed in human embryonic stem cells
Contributor(s) De Iaco A, Planet E
Citation(s) 28459456
Submission date Jan 31, 2017
Last update date May 15, 2019
Contact name Evarist Planet
Organization name EPFL
Street address Route Cantonale
City Lausanne
State/province Vaud
ZIP/Postal code 1015
Country Switzerland
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (2)
GSM2473138 021216_E_S4
GSM2473139 281116_A_S1
This SubSeries is part of SuperSeries:
GSE94325 A family of double-homeodomain transcription factors promotes zygotic genome activation in placental mammals.
BioProject PRJNA369390
SRA SRP098641

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE94322_RAW.tar 840.0 Kb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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