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Series GSE94300 Query DataSets for GSE94300
Status Public on Apr 09, 2018
Title MTF2 recruits Polycomb Repressive Complex 2 by helical shape-selective DNA binding
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Polycomb repression of gene expression is essential for development, with a pivotal role played by trimethylation of histone H3 lysine 27 (H3K27me3) that is deposited by Polycomb Repressive Complex 2 (PRC2). The mechanism by which PRC2 is recruited to target genes has remained largely elusive, in particular in vertebrates. Here we demonstrate that MTF2, one of the three vertebrate homologs of Drosophila Polycomblike, is a DNA-binding, methylation-sensitive PRC2 recruiter in mouse embryonic stem cells. MTF2 directly binds to DNA and is essential for recruitment of PRC2 both in vitro and in vivo. Genome-wide recruitment of the PRC2 catalytic subunit EZH2 is abrogated in MTF2 knock-out cells, resulting in largely reduced H3K27me3 deposition. MTF2 selectively binds regions with a high density of unmethylated CpGs in a context of reduced helix twist, which distinguishes target from non-target CpG islands. These results demonstrate instructive recruitment of PRC2 to genomic targets by MTF2.
Overall design ChIP-seq for Mtf2, Ezh2, H3K27me3, H3K4me3, Jarid2 and input for reference performed in WT, Mtf2, Jarid2 and Eed mutant mouse embryonic stem cells.
Contributor(s) Perino M, van Mierlo G, Karemaker ID, van Genesen S, Vermeulen M, Marks H, van Heeringen SJ, Veenstra GJ
Citation(s) 29808031, 34848733
Submission date Jan 30, 2017
Last update date Jan 07, 2022
Contact name Gert Jan Veenstra
Phone +31 24 3610541
Organization name Radboud University
Department Molecular Developmental Biology
Street address Geert Grooteplein 28
City Nijmegen
State/province Not US or Canada
ZIP/Postal code 6525 GA
Country Netherlands
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (22)
GSM2472741 E14_Ezh2_Rep1
GSM2472742 E14_Ezh2_Rep2
GSM2472743 E14_H3K27me3_Rep1
BioProject PRJNA369274
SRA SRP098595

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Supplementary file Size Download File type/resource
GSE94300_E14Ezh2_MAnorm_unbiased.bed.gz 61.3 Kb (ftp)(http) BED
GSE94300_E14H3K27me3_MAnorm_unbiased.bed.gz 57.3 Kb (ftp)(http) BED
GSE94300_E14H3K4me3_MAnorm_unbiased.bed.gz 234.2 Kb (ftp)(http) BED
GSE94300_E14Mtf2_MAnorm_unbiased.bed.gz 54.7 Kb (ftp)(http) BED
GSE94300_ES_Mtf2GT_Ezh2_MAnorm_unbiased.bed.gz 6.6 Kb (ftp)(http) BED
GSE94300_ES_Mtf2GT_H3K27me3_MAnorm_unbiased.bed.gz 9.0 Kb (ftp)(http) BED
GSE94300_ES_Mtf2GT_H3K4me3_MAnorm_unbiased.bed.gz 230.5 Kb (ftp)(http) BED
GSE94300_RAW.tar 4.4 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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