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Series GSE94280 Query DataSets for GSE94280
Status Public on Mar 31, 2017
Title Regulation of Lipids is Central to Replicative Senescence
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Cellular replicative senescence, a state of permanent cell-cycle arrest that occurs following an extended period of cell division in culture, has been linked to organismal aging, tissue repair and tumorigenesis. In this study, we comparatively investigated the global lipid profiles and mRNA content of proliferating and senescent-state BJ fibroblast cells. We found that both the expression levels of lipid-regulating genes, as well as the abundance of specific lipid families, are actively regulated. We further found that 19 polyunsaturated triacylglycerol species showed the most prominent changes during replicative senescence. We argue that diversion of polyunsaturated fatty acids to glycerolipid biosynthesis could be responsible for the accumulation of specific triacylglycerols. This, in turn, could be one of the cellular mechanisms to prevent lipotoxicity under increased oxidative stress conditions observed during replicative senescence. Collectively, our results place regulation of specific lipid species to a central role during replicative senescence.
Overall design We sequence total RNA from 3 early PD and 3 senesent human BJ cell lines to detect the expressional differences between early PD and senescent cells.
Contributor(s) Lizardo D, Lin Y, Gokcumen O, Atilla-Gokcumen E
Citation(s) 28128379
Submission date Jan 30, 2017
Last update date May 15, 2019
Contact name Omer Gokcumen
Organization name University at Buffalo
Department Biological Sciences
Lab 639
Street address 639 Cooke Hall
City Buffalo
State/province NY
ZIP/Postal code 14260
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (6)
GSM2472225 BO1
GSM2472226 BO2
GSM2472227 BO3
BioProject PRJNA369234
SRA SRP098571

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Supplementary file Size Download File type/resource
GSE94280_DESeq2_results_Y_vs_O.txt.gz 3.2 Mb (ftp)(http) TXT
GSE94280_RAW.tar 15.2 Mb (http)(custom) TAR (of TSV)
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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