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Series GSE9404 Query DataSets for GSE9404
Status Public on Nov 01, 2007
Title Recovery from freezing
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary Screening of genes that respond to cryopreservation stress using yeast DNA microarray.:
We studied the response of yeast cells after cryopreservation treatment using DNA microarray technology. Genes that contribute to "Cell rescue, defense and virulence," "energy," and "metabolism," were significantly induced. These genes were classified as encoding heat shock proteins, oxidative stress scavenger, and enzymes involved in glucose metabolism. The expression profile of mRNA after cryopreservation treatment was calculated to be closer to that following treatment with detergent or plant oils rather than by other stress factors such as heavy metals and agricultural chemicals. These results suggest that the cryopreservation treatment caused damage to the structure of the cell wall and cellular organelles. This was supported by the localization of the products of the induced genes at the cell wall and within cellular organelles.
Keywords: stress response
 
Overall design Series contains triplicate hybridization in each time-point from independent biological samples respectively.
 
Contributor(s) Odani M, Kitagawa E, Iwahashi H
Citation(s) 14580849
Submission date Oct 23, 2007
Last update date Mar 17, 2012
Contact name Emiko Kitagawa
E-mail(s) hitoshi.iwahashi@aist.go.jp
Phone +81-29-861-8508
Fax +81-29-861-8508
Organization name National Institute of Advanced Industrial Science and Technology
Street address Onogawa 16-1
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-8569
Country Japan
 
Platforms (2)
GPL5992 Hitachisoft Hypergene Yeast Chip Ver. 0 (lot 21)
GPL5994 Hitachisoft Hypergene Yeast Chip Ver. 0 (lot 29,30,31)
Samples (9)
GSM239212 Recovery from freezing (15 min) 1st
GSM239213 Recovery from freezing (15 min) 2nd
GSM239214 Recovery from freezing (15 min) 3rd
Relations
BioProject PRJNA103131

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9404_RAW.tar 4.3 Mb (http)(custom) TAR (of GPR)
Raw data included within Sample table
Raw data provided as supplementary file
Processed data included within Sample table

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