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Series GSE94031 Query DataSets for GSE94031
Status Public on Mar 06, 2017
Title Tamoxifen-associated endometrial tumors expose differential enhancer activity for Estrogen Receptor alpha [ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Tamoxifen, a small molecule inhibitor that binds the Estrogen Receptor alpha (ERα), blocks breast cancer progression while increasing the risk for endometrial cancer. In this study, we assessed genome-wide ERα-chromatin interactions in surgical specimens of endometrial tumors from patients who were previously treated for breast cancer with tamoxifen, and endometrial tumors from patients who were treated without tamoxifen. We compared ERα and signal at differential ERα sites in endometrial tumors of nine patients who received tamoxifen with endometrial tumors with six patients who never used tamoxifen. In addition, we performed H3K27ac (a marker for activity) ChIPs on the above mentioned endometrial tumors, and assed this signal at differential ERα sites. Compared to endometrial tumors of non-users, tamoxifen-associated endometrial tumors exposed higher H3K27ac intensities at ERα sites that are enriched in tamoxifen-associated endometrial tumors. Four tamoxifen-associated endometrial tumors that we used in our analysis have been previously published as Tumor A, B, D, and E in GSE81213.
 
Overall design Era and H3K27ac ChIP-Seq
 
Contributor(s) Droog M, Nevedomskaya E, Kim Y, Zwart W
Citation(s) 28167798
Submission date Jan 24, 2017
Last update date May 15, 2019
Contact name Wilbert Zwart
E-mail(s) w.zwart@nki.nl
Organization name Netherlands Cancer Institute
Department Molecular Pathology
Street address Plesmanlaan 121
City Amsterdam
ZIP/Postal code 1066 CX
Country Netherlands
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (24)
GSM2467294 Tumor F ERa
GSM2467295 Tumor G ERa
GSM2467296 Tumor H ERa
This SubSeries is part of SuperSeries:
GSE94524 Tamoxifen-associated endometrial tumors expose differential enhancer activity for Estrogen Receptor alpha
Relations
BioProject PRJNA363085
SRA SRP097718

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE94031_RAW.tar 870.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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