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Series GSE94023 Query DataSets for GSE94023
Status Public on Apr 18, 2017
Title MCF7 breast cancer cell response to estradiol
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The experiment was designed to uncover the response to estradiol (E2) in MCF7 breast cancer cells. The first step was to create a reference sample in a ligand free environment. For that, the cells were placed into estradiol free media for 3 days, which reduced the binding between ER-alpha and E2. The cells were then ready to be re-exposed to E2. Following the introduction of E2, the resultant changes were tracked by multiple ChIP-seq experiments. The experiments were performed at 0, 5, 10, 20, 40, 80, 160, 320, 640 and 1280 minutes after the stimulation. Each ChIP-seq experiment was carried out with a different antibody to measure genome-wide changes in genomic occupancy of their specific protein targets. Specifically, the studied protein factors and histone modifications were: ER-alpha, H3K4me3, and H2AZ. Other previously published data from the same set of experiments are available for Pol-II ChIP-Seq and RNA-Seq (GEO accession GSE62789 and GSE44800).
 
Overall design ChIP-seq using 3 different antibodies at 10 different times after estradiol treatment of MCF7 cells
 
Contributor(s) Dzida T, Iqbal M, Charapitsa I, Matarese F, Stunnenberg HG, Reid G, Honkela A, Rattray M
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Submission date Jan 24, 2017
Last update date May 15, 2019
Contact name H. G. Stunnenberg
E-mail(s) h.stunnenberg@ncmls.ru.nl
Phone +31-24-3610520
Organization name Radboud University
Department Department of Molecular Biology (274)
Street address Geert Grooteplein 28
City Nijmegen
ZIP/Postal code 6525 GA
Country Netherlands
 
Platforms (2)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (29)
GSM2467201 MCF7_E2_10min_H3K4me3
GSM2467202 MCF7_E2_20min_H3K4me3
GSM2467203 MCF7_E2_40min_H3K4me3
Relations
BioProject PRJNA363069
SRA SRP097701

Download family Format
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Supplementary file Size Download File type/resource
GSE94023_RAW.tar 17.9 Gb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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