NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE9377 Query DataSets for GSE9377
Status Public on Oct 18, 2008
Title Endomyocardial biopsies with different acute rejection gradings
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Our goals were to determine whether (i) gene expression patterns in biopsies grouped by acute rejection grade (AR) would be consistent; (ii) a characteristic gene expression profile would be associated with ISHLT Grade 0, 1A & 1B (equivalent to 1R on recently revised criteria) and ISHLT grade 3A & 3B (equivalent to 2R & 3R on recently revised criteria); (iii) gene expression patterns emerging from the analysis would provide insights into mechanisms of AR.
The samples used for gene expression profiling were endomyocardial biopsies (EMB) with different acute rejection gradings. The EMB (n=2726) were obtained from the right site of the interventricular septum of heart transplant recipients (n=2423) and graded according to the guidelines of the International Society for Heart and Lung Transplantation (ISHLT, grade 0, n=9; grade1A, n=5; 1B, n=5; 3A, n=4; 3B, n=43). Selection criteria for inclusion of patients in this analysis required the availability of appropriately stored EMB samples for RNA extraction; absence of any infection that required treatment with an antimicrobial drug at anytime during the posttransplant course of the patient; absence of prior AR rejection of any grade; absence of hemodynamic compromise at the time of EMB; use of standard immunosuppressive regimen; and the absence of HLA antibodies prior to transplant.
 
Overall design An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract.
 
Contributor(s) Holweg C
Citation(s) 21555702
Submission date Oct 19, 2007
Last update date Dec 06, 2012
Contact name Cecile Holweg
E-mail(s) cholweg@cvmed.stanford.edu
Phone 725 3775
Fax 725 1599
Organization name Stanford University
Department Cardiovascular Medicine
Lab Falk CVRC
Street address 300 Pasteur Drive
City Stanford
State/province CA
ZIP/Postal code 94305-5406
Country USA
 
Platforms (1)
GPL887 Agilent-012097 Human 1A Microarray (V2) G4110B (Feature Number version)
Samples (26)
GSM238702 AR_HTx_EMB6935
GSM238703 AR_HTx_EMB1817
GSM238705 AR_HTx_EMB6931
Relations
BioProject PRJNA103085

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9377_RAW.tar 3.3 Mb (http)(custom) TAR
Raw data included within Sample table
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap