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Series GSE93421 Query DataSets for GSE93421
Status Public on Jan 12, 2017
Title Single-cell RNA-seq of 1.3 million brain cells from E18 mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We have made scRNA-Seq libraries from 1.3 million brain cells from 2 E18 mice, and sequenced each cell to ~18,500 reads/cell.
Overall design Cortex, hippocampus, and subventricular zone were purchased from BrainBits (C57EHCV). They were from 2 E18 C57BL/6 mice dissected on the same day, shipped overnight on ice, and stored at 4C until being prepared for scRNA-Seq. Brain tissues were dissociated following the Demonstrated Protocol for Mouse Embryonic Neural Tissue ( 69 scRNA-Seq libraries were made from first mouse brain 2 days after the dissection. Another 64 scRNA-Seq libraries were made from second mouse brain 6 days after the dissection.
Contributor(s) Zheng G
Citation(s) 28091601
Submission date Jan 11, 2017
Last update date May 15, 2019
Contact name Grace X.Y. Zheng
Organization name 10X Genomics Inc
Street address 7068 Koll Center Parkway, Suite # 401
City Pleasanton
State/province California
ZIP/Postal code 94566
Country USA
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (133)
GSM2453043 E18_20160930_Neurons_Sample_01
GSM2453044 E18_20160930_Neurons_Sample_02
GSM2453045 E18_20160930_Neurons_Sample_03
BioProject PRJNA360949
SRA SRP096558

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Supplementary file Size Download File type/resource
GSE93421_brain_aggregate_matrix.hdf5 3.9 Gb (ftp)(http) HDF5
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Raw data are available in SRA
Processed data are available on Series record

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