NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE93374 Query DataSets for GSE93374
Status Public on Jan 31, 2017
Title A Molecular Census of Arcuate Hypothalamus and Median Eminence Cell Types
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Drop-seq and single cell sequencing of mouse arcuate nucleus and median eminence.

Please see below link for searchable cluster-based gene expression.
 
Overall design Drop-Seq was performed on six separate days using mice in C57BL6/J background at various ages/sex noted. On day 1, Chow_1 replicate was obtained. On day 2, Chow_2 replicate was obtained. On day 3, Chow_3 replicate was obtained. On day 4, 10% Diet_1 and HFD_1 replicates were obtained. On day 5, Fast_1 and Refed_1 replicates were obtained. On day 6, Fast_2, Fast_3, Chow_4, and Chow_5 replicates were obtained
Web link https://portals.broadinstitute.org/single_cell/study/a-molecular-census-of-arcuate-hypothalamus-and-median-eminence-cell-types
 
Contributor(s) Tsai LT, Campbell JN
Citation(s) 28166221
Submission date Jan 10, 2017
Last update date Mar 18, 2019
Contact name Linus Tzu-Yen Tsai
E-mail ltsai@bidmc.harvard.edu
Organization name Beth Israel Deaconess Medical Center
Department Medicine, Division of Endocrinology, Diabetes, and Metabolism
Street address 330 Brookline Avenue
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (11)
GSM2452122 Drop-Seq analysis of Pooled Arc-ME single-cell suspension_ad lib Chow_1
GSM2452123 Drop-Seq analysis of Pooled Arc-ME single-cell suspension_ad lib Chow_2
GSM2452124 Drop-Seq analysis of Pooled Arc-ME single-cell suspension_ad lib Chow_3
Relations
BioProject PRJNA360829
SRA SRP096361

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE93374_Merged_all_020816_BatchCorrected_LNtransformed_doubletsremoved_Data.txt.gz 411.4 Mb (ftp)(http) TXT
GSE93374_Merged_all_020816_DGE.txt.gz 42.2 Mb (ftp)(http) TXT
GSE93374_cell_metadata.txt.gz 136.3 Kb (ftp)(http) TXT
Raw data are available in SRA
Processed data is available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap