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Series GSE93333 Query DataSets for GSE93333
Status Public on Jan 13, 2017
Title Integrated functional genomics and craniofacial morphogenesis within the FaceBase Consortium: Alk5 and TGFBR2 mutants
Organism Mus musculus
Experiment type Expression profiling by array
Summary Congenital malformations in facial bones significantly impact the overall representation of the face. Establishing correlations between gene expression and morphogenesis of craniofacial structures may lead to new discoveries of molecular mechanisms of craniofacial development. Thus in the present investigation, we will generate gene expression profiles of facial bones at embryo stage 14.5 to establish their roles in regulating craniofacial development.

This SuperSeries is composed of the SubSeries listed below.
 
Overall design To perform global gene expression profiling analysis of mandible and maxilla development and integrate these datasets with cell lineage and quantitative 3D dynamic imaging analyses. In collaboration with the ontology group within the FaceBase consortium, we will define anatomical landmarks and morphometric parameters of the developing mandible and maxilla. Wnt1cre;Alk5fl/+ mice were mated with Alk5fl/fl mice and pregnant females were sacrificed at embryonic day 14.5 (E14.5), with observance of a vaginal plug denoting E0. Mutant embryos were identified by the presence of cleft palate and calvarial defect. The mandible and maxilla were dissected away from mutant embryos and control litter mates, then each mandible and maxilla was divided into its distal and proximal ends. For the TGFBR2 mutant, essentially the same protocol was used except that Wnt1cre;Tgfbr2fl/+ mice were mated with Tgfbr2fl/fl mice.
 
Contributor(s) Chai Y, Parada C, Grimaldi A, Ho TV, Harunaga J, Samuels B, Punj V
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Submission date Jan 09, 2017
Last update date Feb 28, 2019
Contact name Michael Kesling
E-mail michael.kesling@alumni.stanford.edu
Phone 415-325-4073
Organization name University of Southern California
Street address 2250 Alcazar Street
City Los Angeles
ZIP/Postal code 90033
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (152)
GSM2392863 Mouse-14.5 Control_Tgfbr2 D_Maxilla_Rep1
GSM2392864 Mouse-14.5 Control_Tgfbr2 D_Maxilla_Rep2
GSM2392865 Mouse-14.5 Control_Tgfbr2 D_Maxilla_Rep3
This SuperSeries is composed of the following SubSeries:
GSE89904 Integrated functional genomics and craniofacial morphogenesis within the FaceBase Consortium: TGFBR2 mutant
GSE92420 Integrated functional genomics and craniofacial morphogenesis within the FaceBase Consortium: Alk5 mutant
GSE121193 Integrated functional genomics and craniofacial morphogenesis within the FaceBase Consortium: Alk5 mutant (or control) at embryo stage E12.5
Relations
BioProject PRJNA360626

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE93333_RAW.tar 587.3 Mb (http)(custom) TAR (of CEL)

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