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Series GSE93313 Query DataSets for GSE93313
Status Public on Feb 03, 2017
Title Expression Analysis of 2D, 3D Quiescent, and 3D Activated Pancreatic Stellate Cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary This study used Illumina single-end RNA-sequencing to examine gene expression differences between 2 mouse-derived pancreatic stellate cell lines (PSC4, PSC5) grown either in 2D monolayers, as 3D quiescent cultures, or as 3D activated transwell cocultures with 2 mouse tumor-derived pancreatic ductal organoid lines (T4, T5). Mouse pancreatic stellate cell (PSC) lines were derived from the pancreata of wild-type C57Bl/6J mice. Mouse tumor organoid lines were derived from mouse pancreata containing pancreatic ductal adenocarcinoma (PDAC) from the KrasLSL-G12D; Trp53LSL-R162H; Pdx1-Cre mouse model. We measured genes differentially expressed among 2D, quiescent, and 3D activated PSCs that may reflect the expression changes of heterogeneous CAF population in pancreatic tumors.
 
Overall design Gene expression comparison of 2 murine pancreatic stellate cell lines grown either in 2D monolayers, 3D quiescent cultures, or 3D activated transwell cocultures with 2 murine pancreatic ductal adenocarcinoma-derived pancreatic ductal organoid lines.
 
Contributor(s) Tuveson DA, Baker LA, Handly-Santana A
Citation(s) 28232471
Submission date Jan 09, 2017
Last update date May 15, 2019
Contact name David A. Tuveson
Organization name Cold Spring Harbor Laboratory
Department Cancer Center
Lab The Tuveson Laboratory
Street address 1 Bungtown Rd.
City Cold Spring Harbor
State/province NY
ZIP/Postal code 11724
Country USA
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (8)
GSM2450835 PSC4 2D
GSM2450836 PSC5 2D
GSM2450837 PSC4 3D
Relations
BioProject PRJNA360599
SRA SRP096327

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE93313_DESeq_2Dvs3D.txt.gz 962.9 Kb (ftp)(http) TXT
GSE93313_DESeq_2DvsTranswell.txt.gz 997.1 Kb (ftp)(http) TXT
GSE93313_DESeq_3DvsTranswell.txt.gz 1006.1 Kb (ftp)(http) TXT
GSE93313_countTable.txt.gz 277.2 Kb (ftp)(http) TXT
GSE93313_countTable_normalized.txt.gz 896.6 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data is available on Series record

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