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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 11, 2017 |
| Title |
Loss of a mammalian circular RNA locus causes miRNA deregulation and affects brain function [miRNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Hundreds of circular RNAs (circRNAs) are highly abundant in the mammalian brain, often with conserved expression. Here we show that the circRNA Cdr1as is massively bound by the microRNAs (miRNAs) miR-7 and miR-671 in human and mouse brains. When the Cdr1as locus was removed from the mouse genome, knockout animals displayed impaired sensorimotor gating-a deficit in the ability to filter out unnecessary information-which is associated with neuropsychiatric disorders. Electrophysiological recordings revealed dysfunctional synaptic transmission. Expression of miR-7 and miR-671 was specifically and posttranscriptionally misregulated in all brain regions analyzed. Expression of immediate early genes such as Fos, a direct miR-7 target, was enhanced in Cdr1as-deficient brains, providing a possible molecular link to the behavioral phenotype. Our data indicate an in vivo loss-of-function circRNA phenotype and suggest that interactions between Cdr1as and miRNAs are important for normal brain function.
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| Overall design |
We performed miRNA sequencing for three knockout and three wild type replicates in four brain regins - cerebellum, cortex, hippocampus (2 KO replicates) and olfactory bulb.
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| Contributor(s) |
Piwecka M, Glažar P, Hernandez-Miranda L, Memczak S, Filipchyk A, Klironomos F, Kühn R, Kocks C, Birchmeier C, Rajewsky N |
| Citation(s) |
28798046 |
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| Submission date |
Jan 04, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Petar Glazar |
| Organization name |
Max Delbrück Center for Molecular Medicine
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| Department |
Systems Biology of Gene Regulatory Elements
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| Lab |
Rajewsky lab
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| Street address |
Robert Rössle Str. 10 (H. 87)
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| City |
Berlin |
| ZIP/Postal code |
13092 |
| Country |
Germany |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (23)
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| GSM2445237 |
cerebellum, KO rep1 |
| GSM2445238 |
cerebellum, KO rep2 |
| GSM2445239 |
cerebellum, KO rep3 |
| GSM2445240 |
cortex, WT rep1 |
| GSM2445241 |
cortex, WT rep2 |
| GSM2445242 |
cortex, WT rep3 |
| GSM2445243 |
cortex, KO rep1 |
| GSM2445244 |
cortex, KO rep2 |
| GSM2445245 |
cortex, KO rep3 |
| GSM2445246 |
hippocampus, WT rep1 |
| GSM2445247 |
hippocampus, WT rep2 |
| GSM2445248 |
hippocampus, WT rep3 |
| GSM2445249 |
hippocampus, KO rep2 |
| GSM2445250 |
hippocampus, KO rep3 |
| GSM2445251 |
olfactory bulb, WT rep1 |
| GSM2445252 |
olfactory bulb, WT rep2 |
| GSM2445253 |
olfactory bulb, WT rep3 |
| GSM2445254 |
olfactory bulb, KO rep1 |
| GSM2445255 |
olfactory bulb, KO rep2 |
| GSM2445256 |
olfactory bulb, KO rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE93130 |
Loss of a mammalian circular RNA locus causes miRNA deregulation and affects brain function |
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| Relations |
| BioProject |
PRJNA360107 |
| SRA |
SRP096019 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE93129_RAW.tar |
180.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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