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Series GSE9311 Query DataSets for GSE9311
Status Public on Oct 24, 2007
Title Gene expression in roots and shoots of plants grown on selenate
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Selenate is chemically similar to sulfate and can be taken up and assimilated by plants via the same transporters and enzymes. In contrast to many other organisms, selenium (Se) has not been shown to be essential for higher plants. In excess, Se is toxic and restricts development. Both Se deficiency and toxicity pose problems worldwide. To obtain better insight into the effects of Se on plant metabolism and into plant mechanisms involved in Se tolerance, the transcriptome of Arabidopsis plants grown with or without selenate was studied, and Se-responsive genes identified. Roots and shoots exhibited different Se-related changes in gene regulation and metabolism. Many genes involved in sulfur (S) uptake and assimilation were upregulated. Accordingly, Se treatment enhanced sulfate levels in plants, but the quantity of organic S metabolites decreased. Transcripts regulating the synthesis and signaling of ethylene and jasmonic acid were also upregulated by Se. Selenate appeared to repress plant development, as suggested by the down-regulation of genes involved in cell wall synthesis and auxin-regulated proteins. The Se-responsive genes discovered in this study may help create plants that can better tolerate and accumulate Se, which may enhance the effectiveness of Se phytoremediation or serve as Se-fortified food.
Keywords: selenate, abiotic stress
 
Overall design Arabidopsis thaliana (ecotype WS) plants were vertically grown on +/- 40 micromolar selenate MS medium. Root and shoot tissues of 10-day-old plants were seperated and used for RNA extraction and hybridization on Affymetrix microarrays. All conditions were duplicated.
 
Contributor(s) Van Hoewyk D, Takahashi H, Inoue E, Tamaoki M, Hess A, Pilon-Smits E
Citation(s) 18251864
Submission date Oct 12, 2007
Last update date Aug 28, 2018
Contact name Douglas Van Hoewyk
E-mail dougvh@lamar.colostate.edu
Phone 970 472 5256
Fax 970 472 5256
Organization name Colorado State University
Department Biology Department
Lab Pilon-Smits
Street address 1521 Crestmore PL
City Fort Collins
State/province CO
ZIP/Postal code 80521
Country USA
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (8)
GSM237280 Root control rep 1
GSM237281 Root control rep 2
GSM237282 Root Selenate rep 1
Relations
BioProject PRJNA102967

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Supplementary file Size Download File type/resource
GSE9311_RAW.tar 26.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
Raw data provided as supplementary file

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