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Series GSE9302 Query DataSets for GSE9302
Status Public on Oct 12, 2007
Title A perturbation in the system leads to period doubling
Platform organisms Schizosaccharomyces pombe; Saccharomyces cerevisiae
Sample organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary Perturbation of the gated-synchrony system in yeast with phenelzine, an antidepressant drug used in the treatment of affective disorders in humans, leads to a rapid lengthening in the period of the genome-wide transcriptional oscillation. The effect is a concerted, genome-scale change in expression that is first seen in genes maximally expressed in the late-reductive phase of the cycle, doubling the length of the reductive phase within two cycles after treatment. Clustering of genes based on their temporal patterns of expression yielded just three super clusters whose trajectories through time could then be mapped into a simple 3D figure. In contrast to transcripts in the late-reductive phase, most transcripts do not show transients in expression relative to others in their temporal cluster but change their period in a concerted fashion. Mapping the trajectories of the transcripts into low-dimensional surfaces that can be represented by simple systems of differential equations provides a readily testable model of the dynamic architecture of phenotype. In this system, period doubling may be a preferred pathway for phenotypic change. As a practical matter, low-amplitude, genome-wide oscillations, a ubiquitous but often unrecognized attribute of phenotype, could be a source of seemingly intractable biological noise in microarray studies.
Keywords: Time Course Experiment
Overall design Phenelzine was added to the system after the first cycle to induce period doubling
Contributor(s) Klevecz RR, Li CM
Citation(s) 17043222
Submission date Oct 11, 2007
Last update date Feb 21, 2017
Contact name Robert R Klevecz
Phone 6263018348
Organization name Beckman Research Institute of the City of Hope
Department Biology
Lab Cellular Dynamics
Street address 1450 E. Duarte Rd.
City Duarte
State/province CA
ZIP/Postal code 91010
Country USA
Platforms (1)
GPL2529 [Yeast_2] Affymetrix Yeast Genome 2.0 Array
Samples (48)
GSM237019 IFO0233 Control 13.833 hours (A059_A_Yeast-2_01-1)
GSM237020 IFO0233 Control 13.9 hours (A060_A_Yeast-2_01-1)
GSM237021 IFO0233 Control 13.967 hours (A061_A_Yeast-2_01-1)
BioProject PRJNA102951

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Supplementary file Size Download File type/resource
GSE9302_RAW.tar 49.2 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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