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| Status |
Public on Mar 12, 2019 |
| Title |
Transcriptomic analysis of strains evolved for anaerobic xylose utilization [Azf1] |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Engineering microbes with novel metabolic properties is a critical step for production of biofuels and biochemicals. Synthetic biology enables identification and engineering of metabolic pathways into microbes; however, knowledge of how to reroute cellular regulatory signals and metabolic flux remains lacking. Here we used network analysis of multi-omic data to dissect the mechanism of anaerobic xylose fermentation, a trait important for biochemical production from plant lignocellulose. We compared transcriptomic, proteomic, and phosphoproteomic differences across a series of strains evolved to ferment xylose under various conditions.
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| |
|
| Overall design |
RNA-seq and transcriptome analysis of Azf1 deletion and over-expression (via MoBY 2.0 plasmid) in YPX -O2. Duplicate samples were collected on different days.
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| |
|
| Contributor(s) |
Myers K, Gasch A |
| Citation(s) |
30856163 |
| |
| Submission date |
Dec 24, 2016 |
| Last update date |
Jun 11, 2019 |
| Contact name |
Kevin S Myers |
| E-mail(s) |
kmyers2@wisc.edu
|
| Organization name |
University of Wisconsin - Madison
|
| Department |
Great Lakes Bioenergy Research Center
|
| Street address |
5127 WEI, 1552 University Ave
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53726 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17342 |
Illumina HiSeq 2500 (Saccharomyces cerevisiae) |
|
| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE92908 |
Transcriptomic analysis of strains evolved for anaerobic xylose utilization |
|
| Relations |
| BioProject |
PRJNA358772 |
| SRA |
SRP095641 |
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