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Series GSE92879 Query DataSets for GSE92879
Status Public on Dec 24, 2016
Title Chromosome loop control of oncogenic MYC produces a common vulnerability in diverse cancers [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The majority of human cancers become highly dependent on a deregulated MYC oncogene, thus identifying a common mechanism underlying MYC regulation could provide valuable targets for therapy. We show here that diverse tumor-specific super-enhancers acquired throughout the 3Mb MYC insulated neighborhood functionally interact with a single conserved site occupied by CTCF protein in the MYC promoter. CRISPR-mediated deletion analysis shows that this common CTCF site is required for super-enhancer looping to the MYC promoter, high MYC expression and rapid cell proliferation in multiple cancers. Targeted methylation of the MYC enhancer anchor by a dCAS9-DNMT3A-3L fusion protein abrogates CTCF binding with consequent loss of MYC expression, suggesting a common vulnerability and a novel approach for therapeutic targeting of aggressive cancers.
 
Overall design Evaluation of CTCF or H3K27Ac ChIP-seq in three cell types
 
Contributor(s) Schuijers J
Citation(s) 29641996
Submission date Dec 23, 2016
Last update date May 15, 2019
Contact name Richard A Young
E-mail(s) young_computation@wi.mit.edu
Phone 617-258-5219
Organization name Whitehead Institute for Biomedical Research
Lab Young Lab
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (5)
GSM2439222 HEK293_H3K27Ac
GSM2439223 K562 WT CTCF ChIP
GSM2439224 K562-Input
This SubSeries is part of SuperSeries:
GSE92881 Chromosome loop control of oncogenic MYC produces a common vulnerability in diverse cancers
Relations
BioProject PRJNA358712
SRA SRP095609

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE92879_RAW.tar 435.6 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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