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| Status |
Public on Jul 12, 2018 |
| Title |
GRHL2-dependent enhancer switching maintains a pluripotent stem cell transcriptional subnetwork after exit from naïve pluripotency [ChIP-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
During early development, pluripotent cells of the epiblast show extensive rewiring of enhancers with little associated change in gene expression. The mechanisms underlying and purpose of this rewiring are largely unknown. Here we identified a transcription factor, GRHL2, that is both necessary and sufficient to activate latent enhancers during the transition from naïve embryonic stem cells (ESC) to primed epiblast cells (EpiC). GRHL2 is necessary to maintain expression of its targets in EpiCs. However, these genes are already expressed at equivalent levels in ESCs, suggesting these genes switch enhancer usage during the transition. Identification of alternative enhancers driving these genes in ESCs uncovered an enrichment for the ESC-specific KLF transcription factors. While many KLF targets remain expressed in EpiCs, GRHL2 only regulates a specific subset promoting an epithelial program. These data suggest a model where a large naïve-specific transcriptional network is partitioned into smaller networks to uncouple their regulation in EpiCs, providing more flexibility in gene regulation during lineage specification.
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| Overall design |
ATAC-seq in wildtype embryonic stem cells (ESCs) and epiblast cells (EpiCs); ATAC-seq and ChIP-seq for GRHL2, H3K4me1, H3K27Ac, and SMC1 in wildtype and GRHL2 knockout EpiCs; ChIP-seq for HA-tagged GRHL2, H3K4me1, H3K27Ac, and SMC1 in dox-inducible HA-GRHL2 ESCs and untargeted ESCs both treated with doxycyline for 24hrs.
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| Contributor(s) |
Chen A, Blelloch R |
| Citation(s) |
30017589 |
| |
| Submission date |
Dec 22, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Amy Chen |
| E-mail(s) |
amyfanchen@gmail.com
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| Organization name |
Stanford University
|
| Street address |
279 Campus Drive West
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (56)
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| This SubSeries is part of SuperSeries: |
| GSE93147 |
GRHL2-dependent enhancer switching maintains a pluripotent stem cell transcriptional subnetwork after exit from naïve pluripotency |
|
| Relations |
| BioProject |
PRJNA358507 |
| SRA |
SRP095524 |
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