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Series GSE92699 Query DataSets for GSE92699
Status Public on Sep 11, 2017
Title Distinct mesenchymal lineages and niches promote epithelial self-renewal and myofibrogenesis in the lung
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Using flourescence activated cell sorting, we gated on cells negative for CD45, CD326 and CD31, from single cell suspension generated from Adult mice. We then isolated subsets of YFP-, GFP- or TdTomato expressing cells from the indicated reporter mice. tdTomato indicates Wnt-responsive cells, GFP reports Pdgfr-alpha expression and YFP- was used to isolate lineaged Wnt2-positive cells. Additionally we included cells that were negative for the aforementioned reporters. We performed RNAseq on biological duplicates for each subset.
Overall design Adult Axin2CreERT2:tdT:PdgfraEGFP and Wnt2CreERT2:R26REFYP mice lungs were FACS sorted into five corresponding lineages for RNAseq.
Contributor(s) Zepp JA, Morley MP, Morrisey EE
Citation(s) 28886382
Submission date Dec 21, 2016
Last update date May 15, 2019
Contact name Michael Patrick Morley
Phone 215-898-2026
Organization name Perelman School of Medicine at the University of Pennsylvania
Department Penn Cardiovascular Institute
Street address 3400 Civic Center Blvd, Bldg 421
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (10)
GSM2435500 Negative_0
GSM2435501 Axin2:Pdgfra Rep1
GSM2435502 Axin2 Rep1
BioProject PRJNA358398
SRA SRP095461

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Supplementary file Size Download File type/resource
GSE92699_RAW.tar 1.1 Mb (http)(custom) TAR (of CSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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