|
| Status |
Public on Jan 24, 2017 |
| Title |
Simultaneous transcriptional and epigenomic profiling from specific cell types within heterogeneous tissues in vivo |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
This study introduces “NuTRAP”, a transgenic mouse that allows simultaneous isolation of cell type-specific translating mRNA and chromatin from complex tissues
Using NuTRAP, we successfully characterize gene expression and epigenomic states of various adipocyte populations in vivo,
To demonstrate general usefulness, we chateraterize histone states of in vivo hepatocytes.
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| |
|
| Overall design |
We demonstrate the robustness of NuTRAP-based ChIP-seq by performing titration experiments
|
| |
|
| Contributor(s) |
Roh H, Rosen E |
| Citation(s) |
28122230 |
| |
| Submission date |
Dec 19, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Evan Rosen |
| E-mail(s) |
erosen@bidmc.harvard.edu
|
| Organization name |
Beth Israel Deconess Medical Center
|
| Department |
Endocrinology
|
| Lab |
Rosen Lab
|
| Street address |
3 Blackfan Cir
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL16417 |
Illumina MiSeq (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (36)
|
|
| Relations |
| BioProject |
PRJNA358099 |
| SRA |
SRP095371 |
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