|
| Status |
Public on Jan 13, 2017 |
| Title |
Integrated functional genomics and craniofacial morphogenesis within the FaceBase Consortium: Alk5 mutant |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Congenital malformations in facial bones significantly impact the overall representation of the face. Establishing correlations between gene expression and morphogenesis of craniofacial structures may lead to new discoveries of molecular mechanisms of craniofacial development. Thus in the present investigation, we will generate gene expression profiles of facial bones at embryo stage 14.5 to establish their roles in regulating craniofacial development.
|
| |
|
| Overall design |
To perform global gene expression profiling analysis of mandible and maxilla development and integrate these datasets with cell lineage and quantitative 3D dynamic imaging analyses. In collaboration with the ontology group within the FaceBase consortium, we will define anatomical landmarks and morphometric parameters of the developing mandible and maxilla. Wnt1cre;Alk5fl/+ mice were mated with Alk5fl/fl mice and pregnant females were sacrificed at embryonic day 14.5 (E14.5), with observance of a vaginal plug denoting E0. Mutant embryos were identified by the presence of cleft palate and calvarial defect. The mandible and maxilla were dissected away from mutant embryos and control litter mates, then each mandible and maxilla was divided into its distal and proximal ends.
|
| |
|
| Contributor(s) |
Chai Y, Parada C, Grimaldi A, Ho TV, Harunaga J, Samuels B, Punj V |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 14, 2016 |
| Last update date |
Feb 28, 2019 |
| Contact name |
Michael Kesling |
| E-mail(s) |
michael.kesling@alumni.stanford.edu
|
| Phone |
415-325-4073
|
| Organization name |
University of Southern California
|
| Street address |
2250 Alcazar Street
|
| City |
Los Angeles |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (24)
|
| GSM2429543 |
Mouse 14.5 Control Alk5 D_Mandible _Rep1 |
| GSM2429544 |
Mouse 14.5 Control Alk5 D_Mandible _Rep2 |
| GSM2429545 |
Mouse 14.5 Control Alk5 D_Mandible _Rep3 |
| GSM2429546 |
Mouse 14.5 Control Alk5 P_Mandible_Rep1 |
| GSM2429547 |
Mouse 14.5 Control Alk5 P_Mandible _Rep2 |
| GSM2429548 |
Mouse 14.5 Control Alk5 P_Mandible _Rep3 |
| GSM2429549 |
Mouse 14.5 Control Alk5 D_Maxilla_Rep1 |
| GSM2429550 |
Mouse 14.5 Control Alk5 D_Maxilla_Rep2 |
| GSM2429551 |
Mouse 14.5 Control Alk5 D_Maxilla_Rep3 |
| GSM2429552 |
Mouse 14.5 Control Alk5 P_Maxilla_Rep1 |
| GSM2429553 |
Mouse 14.5 Control Alk5 P_Maxilla_Rep2 |
| GSM2429554 |
Mouse 14.5 Control Alk5 P_Maxilla_Rep3 |
| GSM2429555 |
Mouse 14.5 Mutant Alk5 D_Mandible _Rep1 |
| GSM2429556 |
Mouse 14.5 Mutant Alk5 D_Mandible _Rep2 |
| GSM2429557 |
Mouse 14.5 Mutant Alk5 D_Mandible _Rep3 |
| GSM2429558 |
Mouse 14.5 Mutant Alk5 P_Mandible _Rep1 |
| GSM2429559 |
Mouse 14.5 Mutantl Alk5 P_Mandible _Rep2 |
| GSM2429560 |
Mouse 14.5 Mutant Alk5 P_Mandible _Rep3 |
| GSM2429561 |
Mouse 14.5 Mutant Alk5 D_Maxilla_Rep1 |
| GSM2429562 |
Mouse 14.5 Mutant Alk5 D_Maxilla_Rep2 |
| GSM2429563 |
Mouse 14.5 Mutant Alk5 D_Maxilla_Rep3 |
| GSM2429564 |
Mouse 14.5 Mutant Alk5 P_Maxilla_Rep1 |
| GSM2429565 |
Mouse 14.5 Mutant Alk5 P_Maxilla_Rep2 |
| GSM2429566 |
Mouse 14.5 Mutant Alk5 P_Maxilla_Rep3 |
|
| This SubSeries is part of SuperSeries: |
| GSE93333 |
Integrated functional genomics and craniofacial morphogenesis within the FaceBase Consortium: Alk5 and TGFBR2 mutants |
|
| Relations |
| BioProject |
PRJNA357510 |
Less...
More...