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Series GSE92347 Query DataSets for GSE92347
Status Public on Dec 14, 2016
Title Mapping of DHT-responsive or -independent AR-binding sites induced by activated Src in prostate cancer cell lines [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Building on the observation that metastatic, castration-resistant prostate cancer (CRPC) correlates with activation of Src-family tyrosine kinases, we showed that the expression of activated Src renders LNCaP androgen-independent. Here, we report on RNA-seq and/or AR ChIP-seq analyses of LNCaP, LNCaP[Src], VCaP, 22Rv1 cells grown in the presence or absence of 10 nM DHT for 16h, or LuCaP35.1 tumors grown in androgen-supplemented vs. castrated mice (androgen-dependent vs. castration-resistant). We identify an 11-gene Src-induced signature found only in CRPC in response to DHT, and moreover, the differentail expression of a subset (DPP4, BCAT1, CNTNAP4, CDH3) correlates with earlier PC metastasis onset and poorer survival.
Overall design AR ChIP-seq using N-20 Ab to chromatin, followed by production of paired-end NGS libraries and sequencing. 9 samples are seqeucned for V-CaP cell line, Ln-CaP cell line and Ln-CaP with Src-527F cell line. Each cell line has 3 samples, they are DHT treated, Control, and input.
Contributor(s) Gelman IH
Citation(s) 28055971
Submission date Dec 13, 2016
Last update date May 15, 2019
Contact name Katerina Gurova
Organization name Roswell Park Cancer Insitute
Street address Elm & Carlton Streets
City Buffalo
State/province NY
ZIP/Postal code 14263
Country USA
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (9)
GSM2427944 V-CaP + DHT
GSM2427945 V-CaP-Control
GSM2427946 V-CaP-Input
This SubSeries is part of SuperSeries:
GSE92576 Mapping of DHT-responsive or -independent AR-binding sites induced by activated Src in prostate cancer cell lines
BioProject PRJNA357273
SRA SRP095038

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Supplementary file Size Download File type/resource
GSE92347_RAW.tar 390.0 Kb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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