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Series GSE9217 Query DataSets for GSE9217
Status Public on Oct 02, 2008
Title Transcriptomes for different level of glucose
Platform organisms Schizosaccharomyces pombe; Saccharomyces cerevisiae
Sample organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary To characterize gene expression that is dependent on the strength of calorie restriction (CR), we obtained transcriptome at different levels of glucose, which is a major energy and carbon source for budding yeast. To faithfully mimic mammalian CR in yeast culture, we reconstituted and grew seeding yeast cells in fresh 2% YPD media before inoculating into 2%, 1%, 0.5% and 0.25% YPD media to reflect different CR strengths. We collected and characterized 160 genes that responded to CR strength based on the rigorous statistical analyses of multiple test corrected ANOVA (adjusted é value < 0.1 or raw é value < 0.0031) and Pearson correlation (|r| > 0.7). Based on the individual gene studies and the GO Term Finder analysis of 160 genes, we found that CR dosedependently and gradually increased mitochondrial function at the transcriptional level. Therefore, we suggest these 160 genes are markers that respond to CR strength and that might be useful in elucidating CR mechanisms, especially how stronger CR extends life span more.
Overall design Different glucose levels: 2%, 1%, 0.5%, and 0.25%
Three biological replicates in each level
Contributor(s) Lee C, Lee Y
Citation(s) 18679056
Submission date Oct 03, 2007
Last update date Feb 21, 2017
Contact name Cheol-Koo Lee
Phone 82-2-3290-3008
Organization name Korea University
Department Biotechnology
Lab Functional Genomics
Street address Korea University, Biotechnology #318 West building, Anam-dong, Seongbuk-gu
City Seoul
ZIP/Postal code 136-701
Country South Korea
Platforms (1)
GPL2529 [Yeast_2] Affymetrix Yeast Genome 2.0 Array
Samples (12)
GSM233176 2% glucose log_2_1
GSM233177 2% glucose log_2_2
GSM233178 2% glucose log_2_3
BioProject PRJNA102809

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Supplementary file Size Download File type/resource
GSE9217_RAW.tar 51.2 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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