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Series GSE9138 Query DataSets for GSE9138
Status Public on Oct 25, 2007
Title PIWI-associated piRNA in Drosophila melanogaster ovary
Organism Drosophila melanogaster
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Heterochromatin, representing the silenced state of transcription, largely consists of transposon-enriched and highly repetitive sequences. Implicated in heterochromatin formation and transcriptional silencing in Drosophila are PIWI and repeat-associated small interfering RNAs (rasiRNAs). Despite this, the role of PIWI in rasiRNA expression and heterochromatic silencing remains unknown. Here we report the identification and characterization of 12,903 PIWI-interacting RNAs (piRNAs) in Drosophila, demonstrating that rasiRNAs represent a subset of piRNAs.
Keywords: PIWI, piRNA, epigenetic regulation, heterochromatin
 
Overall design PIWI-associated small RNA cDNA library was sequenced for one time by high-throughput 454 pyrosequencing. Putative small RNA sequences were extracted and BLAST against the Drosophila melanogaster genome release 5. Presented here is a list of non-redundant PIWI-associated small RNAs, which have at least one genome match determined by BLASTn.
 
Contributor(s) Yin H, Lin H
Citation(s) 17952056
Submission date Sep 23, 2007
Last update date Mar 17, 2012
Contact name Hang Yin
E-mail hang.yin@duke.edu
Phone (203)7856215
Organization name Yale University Stem Cell Center
Department Department of Cell Biology
Lab Haifan Lin laborotary
Street address 10 Amistead Street, Room 220
City New Haven
State/province CT
ZIP/Postal code 06509
Country USA
 
Platforms (1)
GPL5922 PIWI-associated piRNA in Drosophila melanogaster ovary
Samples (1)
GSM231091 PIWI-associated piRNA in Drosophila melanogaster ovary (454 Pyrosequencing, 2006)
Relations
BioProject PRJNA102681

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9138_RAW.tar 5.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Raw data provided as supplementary file

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