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Series GSE91082 Query DataSets for GSE91082
Status Public on Nov 08, 2017
Title The transcription factor Isl1 is associated with multiple signalling pathways important during genital development
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary To identify regulatory targets of the transcription factor Isl1 in the developing mouse genitalia, we performed ChIP-Seq using an anti-ISL1 rabbit monoclonal antibody on pooled chromatin isolated from multiple E14.5 mouse embryonic genital tubercles (GT). Our analysis identified more than 7,054 genomic regions enriched in both of two replicates. The majority of these binding sites are located more than 5 kb from the closest annotated transcriptional start site (TSS), with nearly one third of the binding sites more than 50kb away from the nearest TSS. A de novo motif search using HOMER demonstrated that GT Isl1 ChIP-seq peaks are enriched for a motif that matches the consensus sequence bound by Isl1 in other tissues. When compared to active enhancers in the GT identified using H3K27ac ChIP-Seq, we found that Isl1 binding sites are strongly enriched in the GT compared to other embryonic tissues. Further analysis of the location of Isl1 peaks revealed a strong association with genes implicated in limb and urogenital development. We conclude that there is a strong association between Isl1 regulatory activity and genes involved in genital development.
Overall design Examination of Isl1 transcription factor binding in two replicates of chromatin isolated from the developing genital tubercles of E14.5 mouse embryos using ChIP-Seq.
Contributor(s) Infante CR, Park S, Menke DB
Citation(s) 29126155
Submission date Dec 09, 2016
Last update date May 15, 2019
Contact name Carlos Rafael Infante
Organization name University of Georgia
Department Genetics
Lab Menke
Street address 500 D.W. Brooks Dr
City Athens
State/province GA
ZIP/Postal code 30602
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (4)
GSM2420697 Isl1_ChIP_Replicate1
GSM2420698 Input_DNA_Replicate1
GSM2420699 Isl1_ChIP_Replicate2
BioProject PRJNA356836
SRA SRP094830

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Supplementary file Size Download File type/resource
GSE91082_RAW.tar 1.9 Gb (http)(custom) TAR (of BED, BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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