|
| Status |
Public on May 03, 2017 |
| Title |
CTCF and cohesin regulate chromatin loop stability with distinct dynamics |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The study investigates the nuclear organization and the binding dynamics of CTCF and cohesin by super-resolution microscopy and ChIP-seq. We determined the genome-wide binding profile of CTCF and Rad21 cohesin subunit in wild type mouse ES cells and compared them to a double FLAG-Halo-mCTCF/mRad21-SNAPf-V5 knock-in mES line, generated by CRISPR/Cas9-mediated genome editing to perform imaging experiments.
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| |
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| Overall design |
ChIP-Seq of CTCF and Rad21 cohesin subunit in wild type mouse ES cells (WT) and in mouse ES cells homozygous for FLAG-Halo-CTCF and Rad21-SNAPf-V5 (C59 clone). ChIP-Seq libraries were prepared independently from two ChIP biological replicates, using normal serum IgGs as a control, and including total inputs as reference datasets.
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| Web link |
https://elifesciences.org/content/6/e25776
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| |
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| Contributor(s) |
Sejr Hansen A, Cattoglio C, Pustova I, Tjian R, Darzacq X |
| Citation(s) |
28467304, 31205001 |
| |
| Submission date |
Dec 07, 2016 |
| Last update date |
Jun 24, 2019 |
| Contact name |
Robert Tjian |
| E-mail(s) |
jmlim@berkeley.edu
|
| Phone |
(510) 642-0884
|
| Organization name |
UC Berkeley
|
| Department |
MCB
|
| Lab |
Tjian Lab
|
| Street address |
University of California, Berkeley 450 Li Ka Shing #3370
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (8)
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| Relations |
| BioProject |
PRJNA356611 |
| SRA |
SRP094705 |
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