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Series GSE90958 Query DataSets for GSE90958
Status Public on Sep 18, 2017
Title ncRNA transcription-induced changes in nuclear architecture directs with high precision enhancer-promoter interaction
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Other
Methylation profiling by high throughput sequencing
Summary It is now well established that transcriptional enhancers dictate with high precision lineage-specific programs of gene expression. However, it has remained an enigma as to how distally located enhancers, while separated by vast genomic distances, selectively target their cognate promoters. To explore the mechanism that underpins this precision-directed targeting we have examined the nuclear architecture of a super-enhancer that regulates the expression of Bcl11b. Bcl11b is a transcriptional regulator that specifies T cell fate and suppresses T-cell malignancy. We show that in T cell progenitors the Bcl11b super-enhancer is located at the nuclear lamina in a transcriptionally silent environment. We found that during T cell commitment the super-enhancer repositioned from the lamina to the transcriptionally permissive compartment. In search for candidate factors that reposition the super-enhancer we identified ThymoD (Thymocyte-Differentiation Factor). ThymoD expression pattern in T cell progenitors immediately precedes that of Bcl11b. We show that ThymoD transcription is required to activate Bcl11b expression, to promote T cell fate, and to suppress the development of T cell malignancy. We found that ThymoD acts in cis to reposition the Bcl11b super-enhancer from the lamina to the nuclear interior. We demonstrate that ThymoD brings the Bcl11b super-enhancer and promoter into close spatial proximity by modulating CTCF and cohesin occupancy and chromatin folding across the Bcl11b intergenic region. These data reveal how ncRNA transcription modulates nuclear location and chromatin folding permitting distally located enhancers to select with high precision their cognate promoter regions.
 
Overall design Adult hematopoietic stem cells (aHSCs) were obtained from either WT or ThymoD+/p(A) or ThymoD p(A)/p(A) bone marrow. aHSCs were cultured on OP9-DL1 stromoal cells with Flt3-L and IL7. DN2 cells were sorted by AriaII. 6 ATAC samples from in-vitro cultured DN2 cells (duplicates, WT, ThymoD +/p(A) and ThymoD p(A)/p(A)), 6 RNAseq samples from in-vitro cultured DN2 cells (triplicates, WT, ThymoD p(A)/p(A)), 6 SMC3 ChIP-seq samples from in-vitro cultured DN2 cells (duplicates, WT, ThymoD +/p(A) and ThymoD p(A)/p(A)), 4 CTCF ChIP-seq samples from in-vitro cultured DN2 cells (WT, duplicates ThymoD+/- and ThymoD p(A)/p(A)), 3 H3K4me1 ChIP-seq samples from in-vitro cultured DN2 cells (WT, ThymoD +/p(A) and ThymoD p(A)/p(A)), 3 H3K4me3 ChIP-seq samples from in-vitro cultured DN2 cells (WT, ThymoD+/- and ThymoDp(A)/p(A)), 3 H3.3 ChIP-seq samples from in-vitro cultured DN2 cells (WT, ThymoD+/- and ThymoDp(A)/p(A)), 2 Hi-C samples from in-vitro cultured DN2 cells (WT and ThymoDp(A)/p(A)). This study includes a re-analysis of Hi-C samples GSM987816 and GSM987818 from GSE40173. Tag counts for the re-analyzed HiC samples and the wildtype HiC sample (GSM2418375) were normalized to the sample with lowest sequencing depth. For multipotent progenitors (GSM987816), pro-B (GSM987818) and wild-type DN2 (GSM2418375) comparisons a map resolution of 50 kb was chosen to ensure that > 80% of loci were associated with at least 1000 genomic contacts. The processed data files for the re-analyzed samples are available on the series record.
 
Contributor(s) Isoda T, Moore AJ, Chandra V, Aida M, Denholtz M, He Z, van Hamburg JP, Fisch KM, Chang AN, Murre C
Citation(s) 28938112
Submission date Dec 06, 2016
Last update date Jul 25, 2021
Contact name Kees Murre
E-mail(s) cmurre@ucsd.edu
Organization name UCSD
Department Biology
Lab Murre lab
Street address 9500 gilman drive, NSB 5114A
City La Jolla
State/province CALIFORNIA
ZIP/Postal code 92093
Country USA
 
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (80)
GSM2418338 ATAC DN2 WT 1
GSM2418339 ATAC DN2 WT 2
GSM2418340 ATAC DN2 ThymoD+/p(A) 1
Relations
Reanalysis of GSM987816
Reanalysis of GSM987818
BioProject PRJNA356446
SRA SRP094653

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE90958_DN2_WT_KO_counts.xlsx 1.0 Mb (ftp)(http) XLSX
GSE90958_RAW.tar 8.5 Gb (http)(custom) TAR (of BED, BEDGRAPH, TXT)
GSE90958_preproB.mm9.2r.50kR.100kSR.norm.chr12.100t120.MatFile.txt.gz 459.1 Kb (ftp)(http) TXT
GSE90958_proB.mm9.3ntoB00.50kR.100kSR.norm.chr12.100t120.MatFile.txt.gz 458.4 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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