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| Status |
Public on Jun 21, 2018 |
| Title |
RNA sequencing of ESC/iPSC-derived purified PAX6-GFP neural progenitors form control and Phelan-Mcdermid patients |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this study was to generated and transcriptionally analyze purified populations of Phelan-McDermid and control pluripotent stem cells-derived neural progenitors. This type of analysis allowed us to identify prevoisly unknown transcriptional phenotypic changes in patient neural progenitors which appear to be independent of functional synapses.
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| Overall design |
Patient and control pluripotent stem cells were targeted by CRISPR/Cas9 and a PAX6-GFP reporter plasmid. Correctly modified clones were selected and subsequently differentiated into PAX6 expressing neural progenitors.
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| Contributor(s) |
Jaenisch R, Roessler R |
| Citation(s) |
30456368 |
| Submission date |
Nov 30, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Reinhard Roessler |
| Organization name |
Whitehead Institute
|
| Street address |
455 Main street
|
| City |
Cambridge |
| ZIP/Postal code |
02142 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA355605 |
| SRA |
SRP094413 |
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