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Series GSE90716 Query DataSets for GSE90716
Status Public on Mar 20, 2018
Title Hijacking of stress response machinery by oncogenes in acute leukaemia [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary HSF1 orchestrates the heat shock response pathway. This pathway is co-opted in cancer and provides critical stress relief from oncogenic stress. HSF1 has a diverse occupancy signature depending on the cell type. In this study, we performed HSF1 ChIP-seq analysis using the human T-ALL cell line CUTLL1 and P12. These results revealed the HSF1 chromatin binding signature in CUTLL1 and P12 cells. MYC is a driving oncogene in T-ALL. The non-oncogene addiction pathways that act downstream of this transcription factor to support anabolic pathways are not well-understood. For this reason, we performed MYC ChIP-seq analysis using the human T-ALL cell line CUTLL1. These results revealed that HSF1 and HSF1 targets are included in the MYC binding signature.
 
Overall design Twenty million cells were used for the ChIP and precipitated using 5 micrograms of antibody (cell signaling, 4356) against human HSF1. Twenty million cells were used for the ChIP and precipitated using 5 micrograms of antibody (santa cruz biotechnology, N-262) against human MYC
 
Contributor(s) Kourtis N, Lazaris C
Citation(s) 30038221
Submission date Nov 30, 2016
Last update date May 15, 2019
Contact name Nikos Kourtis
E-mail(s) nkourtis@gmail.com
Organization name NYU School of Medicine
Street address 550 First Avenue
City New York
ZIP/Postal code 10016
Country USA
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (9)
GSM2411132 ChIP-Seq analysis of HSF1 in CUTLL1 cells (rep1)
GSM2411133 ChIP-Seq analysis of HSF1 in CUTLL1 cells (rep2)
GSM2411134 Sequencing analysis of Input (for HSF1 ChIP) in CUTLL1 cells
This SubSeries is part of SuperSeries:
GSE90717 Hijacking of stress response machinery by oncogenes in acute leukaemia
Relations
BioProject PRJNA355575
SRA SRP094123

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SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE90716_RAW.tar 1.3 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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