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Series GSE90559 Query DataSets for GSE90559
Status Public on Mar 07, 2019
Title Sox2 is required for global chromatin interactions in brain-derived neural stem cells [ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Genome wide mapping of Sox2 binding sites in neural progenitor cells
Overall design ChIP-Seq experiment on transcription factor Sox2 in neural progenitor cells. Cells were collected as small neurospheres and fixed either directly ("Spheres") or after dissociation ("Singles"). Cells were fixed sequentially with di(N-succimidyl) glutarate and 1% formaldehyde in phosphate-buffered saline and then lysed, sonicated and immunoprecipitated as described previously (Mateo et al., 2015 and references therein). SOX2 was immunoprecipitated with 3mg of goat anti-SOX2 (Santa Cruz sc-17320).
Contributor(s) Martynoga B
Citation(s) 30849367
Submission date Nov 26, 2016
Last update date May 21, 2019
Contact name Federico Zambelli
Organization name University of Milan
Department Department of Biosciences
Street address Via Giovanni Celoria 26
City Milano
ZIP/Postal code 20133
Country Italy
Platforms (1)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
Samples (4)
GSM2406795 Input DNA_Spheres
GSM2406796 Sox2 ChIP_Spheres
GSM2585546 Input DNA_Singles
This SubSeries is part of SuperSeries:
GSE90561 Sox2 is required for functional chromatin connectivity in brain-derived neural stem cells.
BioProject PRJNA355013
SRA SRP093985

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE90559_Sox2_Singles_Q5_aggregated_peaks.bed.txt.gz 588.6 Kb (ftp)(http) TXT
GSE90559_Sox2_Spheres_Q5_aggregated_summits.bed.txt.gz 227.6 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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