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| Status |
Public on Jul 08, 2017 |
| Title |
Genome-wide Mapping and Analysis of Aryl Hydrocarbon Receptor (AHR) and Aryl Hydrocarbon Receptor Repressor (AHRR) by ChIP-Seq |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
In this study, we compared the genome-wide binding profiles of AHR and AHRR in MCF-7 human breast cancer cells treated for 24 h with TCDD using chromatin immunoprecipitation followed by next generation sequencing (ChIP-Seq). Overall, this study reveals that AHR and AHRR exhibit similar but also distinct genome-wide binding profiles, supporting the notion that AHRR is a context- and gene-specific repressor of AHR activity.
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| |
|
| Overall design |
MCF7 treated with DMSO (vehicle) or TCDD for 24h; ChIP-seq in triplicates using AHR- and AHRR-specific antibodies with Illumina HiSeq 2500
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| |
|
| Contributor(s) |
Yang SY, Ahmed S, Matthews J |
| Citation(s) |
28681081 |
| Submission date |
Nov 26, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Jason Matthews |
| Organization name |
University of Oslo
|
| Department |
Department of Nutrition, Institute of Basic Medical Sciences
|
| Lab |
2201
|
| Street address |
1046 Blindern
|
| City |
Oslo |
| ZIP/Postal code |
0372 Oslo |
| Country |
Norway |
| |
|
| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA355002 |
| SRA |
SRP093977 |
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