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Series GSE89804 Query DataSets for GSE89804
Status Public on Feb 22, 2017
Title Long non-coding RNAs and mRNAs in nasopharyngeal carcinoma metastasis
Organism Homo sapiens
Experiment type Expression profiling by array
Non-coding RNA profiling by array
Summary Increasing evidence has demonstrated a significant role for long non-coding RNAs (lncRNAs) in tumorigenesis. However, their functions in nasopharyngeal carcinoma (NPC) metastasis remain largely unknown. In this study, a model compared high and low metastatic NPC cell lines (5-8F vs. 6-10B and S18 vs. S26)was constructed to determine the expression profile of lncRNAs using the microarray analysis, and we found 167 lncRNAs and 209 mRNAs were differentially expressed. Validationof 26 significantly dysregulated lncRNAs by qRT-PCR showed the expression patterns of 22 lncRNAs were in accordance with the microarray data. Furthermore, the expression level of ENST00000470135, which was the most upregulated lncRNA in high metastatic cell lines, was significantly higher in NPC cell lines and tissues with lymph node metastasis(LNM)and knocking down ENST00000470135 suppressed the migration, invasion and proliferation of NPC cells in vitro. In conclusion, our study revealed expression patterns of lncRNAs in NPC metastasis. The dysregulated lncRNAs may act as novel biomarkers and therapeutic targets for NPC.
Overall design Total RNA recovered from two sets of cell lines (5-8F vs. 6-10B and S18 vs. S26) were used to acquire different expression profiles of mRNAs and lncRNAs in high metastatic potential and low metastatic potential nasopharyngeal carcinoma cell lines.
Contributor(s) Wen X, Ma J, Liu N
Citation(s) 27886062
Submission date Nov 14, 2016
Last update date Mar 12, 2018
Contact name Jun Ma
Organization name Sun Yat-sen University Cancer Center
Street address 651 Dongfeng Road East
City Guangzhou
ZIP/Postal code 510060
Country China
Platforms (1)
GPL16956 Agilent-045997 Arraystar human lncRNA microarray V3 (Probe Name Version)
Samples (4)
GSM2389485 5-8F
GSM2389486 6-10B
GSM2389487 S18
BioProject PRJNA353353

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Supplementary file Size Download File type/resource
GSE89804_RAW.tar 11.0 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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