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| Status |
Public on Apr 19, 2018 |
| Title |
Deep functional analysis of synII, a 770 kb synthetic yeast chromosome |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
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| Summary |
Herein we report the successful design, construction and characterization of a 770 kb synthetic yeast chromosome II (synII). Our study incorporates characterization at multiple levels, including phenomics, transcriptomics, proteomics, chromosome segregation and replication analysis to provide the most thorough and comprehensive analysis of a synthetic chromosome. Our “Trans-Omics” analyses reveal a modest but potentially significant pervasive up-regulation of translational machinery observed in synII is mainly caused by the deletion of 13 tRNAs. By both complementation assays and SCRaMbLE, we targeted and debuged the origin of a growth defect at 37°C in glycerol medium, which is related to misregulation of the HOG response. Despite the subtle differences, the synII strain shows highly consistent biological processes comparable to the native strain.
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| Overall design |
Measurement of genome replication timing from a S. cerevisiae yeast strain with a 770 kb synthetic yeast chromosome II (synII). Two samples were analysed: a replicating sample (from S phase) and a non-replicating sample (from G2 phase).
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| Contributor(s) |
Shen Y, Wang Y, Chen T, Gao F, Gong J, Abramczyk D, Walker R, Zhao H, Chen S, Liu W, Luo Y, Müller CA, Paul-Dubois-Taine A, Alver B, Stracquadanio G, Mitchell LA, Luo Z, Fan Y, Zhou B, Wen B, Tan F, Wang Y, Zi J, Xie Z, Li B, Yang K, Richardson SM, Yuan Y, French CE, Nieduszynski CA, Koszul R, Marston AL, Jiang H, Wang J, Bader JS, Dai J, Boeke JD, Xu X, Cai Y, Yang H |
| Citation(s) |
28280153 |
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| Submission date |
Nov 07, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Conrad A Nieduszynski |
| Organization name |
Earlham Institute
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| Lab |
DNA replication & genome stability
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| Street address |
Norwich Research Park
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| City |
Norwich |
| ZIP/Postal code |
NR4 7UZ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL17342 |
Illumina HiSeq 2500 (Saccharomyces cerevisiae) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA352710 |
| SRA |
SRP092775 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE89611_SynII.wig.gz |
50.1 Kb |
(ftp)(http) |
WIG |
| GSE89611_SynII_genome_complete.fa.gz |
3.6 Mb |
(ftp)(http) |
FA |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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