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| Status |
Public on Nov 04, 2016 |
| Title |
A new model for long-range chromatin reorganization linked to enhancer activation [Agilent_RNA] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by genome tiling array
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| Summary |
The most prominent model for long-range enhancer regulation involves direct enhancer-promoter interaction by looping out the intervening chromatin. Using a synthetic biology approach, we have determined that a chromatin unfolding bteween Shh and its enhancers is regulated specifically by the Shh-Brain-Enhancers and is mediated by the recruitment of Poly (ADP-Ribose) Polymerase 1. This ‘chromatin unfolding’ model represents a new mechanism of long-range enhancer-promoter communication in addition to the looping and tracking models.
RNA-on-chip for probing nuclear RNA on the Shh regulatory region on ESCs and NPC showed no spreading of nuclear RNA in the regulatory region upon Shh activation.
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| Overall design |
Comparison of ESCs and NPC nuclear RNA profiles on Shh region
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| Contributor(s) |
Benabdallah NS, Williamson I, Illingworth RS, Boyle S, Grimes GR, Therizols P, Bickmore WA |
| Citation missing |
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| |
| Submission date |
Nov 03, 2016 |
| Last update date |
Jun 13, 2018 |
| Contact name |
Nezha Suzanne Benabdallah |
| E-mail(s) |
nsbenabdallah@gmail.com
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| Organization name |
University of Edinburgh
|
| Department |
Institute of Genetics and Cancer
|
| Street address |
Crewe Road South
|
| City |
Edinburgh |
| State/province |
Midlothian |
| ZIP/Postal code |
EH4 2XR |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL22635 |
Agilent-076323 En2_2 Custom Tiling Array G4822A (Feature number version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA352421 |
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