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| Status |
Public on Nov 02, 2016 |
| Title |
A new model for long-range chromatin reorganization linked to enhancer activation [5C] |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
The most prominent model for long-range enhancer regulation involves direct enhancer-promoter interaction by looping out the intervening chromatin. Using a synthetic biology approach, we have determined that a chromatin unfolding bteween Shh and its enhancers is regulated specifically by the Shh-Brain-Enhancers and is mediated by the recruitment of Poly (ADP-Ribose) Polymerase 1. This ‘chromatin unfolding’ model represents a new mechanism of long-range enhancer-promoter communication in addition to the looping and tracking models.
Using 5C study the enhancer-driven activation of the Sonic hedgehog gene (Shh)
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| Overall design |
5C oligonucleotides were designed around HindIII restriction sites following an alternative scheme
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| Contributor(s) |
Benabdallah NS, Williamson I, Illingworth RS, Boyle S, Grimes GR, Therizols P, Bickmore WA |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 01, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Nezha Suzanne Benabdallah |
| E-mail(s) |
nsbenabdallah@gmail.com
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| Organization name |
University of Edinburgh
|
| Department |
Institute of Genetics and Cancer
|
| Street address |
Crewe Road South
|
| City |
Edinburgh |
| State/province |
Midlothian |
| ZIP/Postal code |
EH4 2XR |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
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| Samples (12)
|
| GSM2367827 |
NPC norm biological replicate 1 technical replicate2 |
| GSM2367828 |
NPC norm biological replicate 2 technical replicate1 |
| GSM2367829 |
NPC norm biological replicate 2 technical replicate2 |
| GSM2367830 |
tSBE6-Vp64 norm biological replicate 1 |
| GSM2367831 |
tSBE2-Vp64 norm biological replicate 1 |
| GSM2367832 |
tSBE(6+2-Vp64) norm biological replicate 1 technical replicate1 |
| GSM2367833 |
tSBE(6+2-Vp64) norm biological replicate 1 technical replicate2 |
| GSM2367834 |
tSBE(6+2-Vp64) norm biological replicate 2 technical replicate1 |
| GSM2367835 |
tSBE(6+2-Vp64) norm biological replicate 2 technical replicate2 |
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| This SubSeries is part of SuperSeries: |
| GSE89557 |
A new model for long-range chromatin reorganization linked to enhancer activation |
|
| Relations |
| BioProject |
PRJNA352048 |
| SRA |
SRP092399 |
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