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Series GSE89315 Query DataSets for GSE89315
Status Public on Nov 21, 2016
Title Tet proteins influence the balance between neuroectoderm and cardiac mesoderm fate choice by inhibiting Wnt signaling
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary To determine the global distribution of Tet3 DNA binding sites, we performed ChIP-seq in neural precursor cells (NPCs). We report that Tet3-binding sites clustered close to transcription start sites (TSS), with a low frequency of binding at distal regions relative to the TSS. DNA motif analysis identified a CpG-rich sequence as the highest-ranked motif among Tet3-binding sites. GO analysis of Tet3 target genes showed enrichment for genes related to mesoderm development and neural differentiation. IPA analysis showed that Tet3 target genes were also strongly enriched for components of the Wnt signaling pathway.
 
Overall design To determine the global distribution of Tet3 DNA binding sites, we performed ChIP-seq experiments using Tet3 antibodies in neural precursor cells (NPCs).
 
Contributor(s) Li X, Rao A
Citation(s) 27930333
Submission date Oct 30, 2016
Last update date May 15, 2019
Contact name ANJANA RAO
E-mail(s) arao@lji.org
Organization name La Jolla Institute for Allergy and Immunology
Street address 9420 Athena Circle
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platforms (1)
GPL16790 AB 5500 Genetic Analyzer (Mus musculus)
Samples (2)
GSM2365904 Tet3 ChIPSeq
GSM2365905 Input DNA
This SubSeries is part of SuperSeries:
GSE89316 Tet proteins influence the balance between neuroectodermal and mesodermal fate choice by inhibiting Wnt signaling
Relations
BioProject PRJNA351755
SRA SRP092293

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89315_RAW.tar 60.0 Kb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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